Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16347 | 3' | -53 | NC_004084.1 | + | 11345 | 0.66 | 0.846616 |
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Target: 5'- --aGCUCggUCGAGC--CGGuUCGAGUCGa -3' miRNA: 3'- ugaCGAG--AGUUCGuaGCC-AGCUCAGU- -5' |
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| 16347 | 3' | -53 | NC_004084.1 | + | 21388 | 0.67 | 0.790099 |
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Target: 5'- -gUGCcagCUCGGGCAUgaucCGGUCGAcGUCc -3' miRNA: 3'- ugACGa--GAGUUCGUA----GCCAGCU-CAGu -5' |
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| 16347 | 3' | -53 | NC_004084.1 | + | 9172 | 0.68 | 0.727444 |
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Target: 5'- -aUGCcgCUCGAGCcuUCGGaugUCGAGUCu -3' miRNA: 3'- ugACGa-GAGUUCGu-AGCC---AGCUCAGu -5' |
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| 16347 | 3' | -53 | NC_004084.1 | + | 54074 | 0.68 | 0.694616 |
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Target: 5'- cGCUGUUcCUC-GGCGUCGGUgGAGa-- -3' miRNA: 3'- -UGACGA-GAGuUCGUAGCCAgCUCagu -5' |
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| 16347 | 3' | -53 | NC_004084.1 | + | 18845 | 0.7 | 0.627572 |
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Target: 5'- cGCgGCUUUCGucgacGGUccCGGUCGAGUCGa -3' miRNA: 3'- -UGaCGAGAGU-----UCGuaGCCAGCUCAGU- -5' |
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| 16347 | 3' | -53 | NC_004084.1 | + | 10021 | 1.08 | 0.001836 |
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Target: 5'- gACUGCUCUCAAGCAUCGGUCGAGUCAc -3' miRNA: 3'- -UGACGAGAGUUCGUAGCCAGCUCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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