Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16351 | 3' | -53.4 | NC_004084.1 | + | 22028 | 0.67 | 0.768152 |
Target: 5'- cCGAGCGAGaGGaUCGaacgguagaGCUCGGGUu- -3' miRNA: 3'- -GCUCGCUCaCC-AGCaa-------UGAGCCCAuc -5' |
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16351 | 3' | -53.4 | NC_004084.1 | + | 53243 | 0.68 | 0.757956 |
Target: 5'- aCGAGCGGGcGGUCGUcgACggcgacgagaUCGGGccGGg -3' miRNA: 3'- -GCUCGCUCaCCAGCAa-UG----------AGCCCa-UC- -5' |
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16351 | 3' | -53.4 | NC_004084.1 | + | 52038 | 0.68 | 0.726643 |
Target: 5'- uGAGCGAGcGGUCuacgGCgUCGGGaUGGa -3' miRNA: 3'- gCUCGCUCaCCAGcaa-UG-AGCCC-AUC- -5' |
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16351 | 3' | -53.4 | NC_004084.1 | + | 8119 | 1.08 | 0.002228 |
Target: 5'- uCGAGCGAGUGGUCGUUACUCGGGUAGc -3' miRNA: 3'- -GCUCGCUCACCAGCAAUGAGCCCAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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