Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16352 | 3' | -62.4 | NC_004084.1 | + | 10451 | 0.73 | 0.151829 |
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Target: 5'- cCGGaaGCCCGCGAgGCGa-UCCAGGACGg -3' miRNA: 3'- -GCCg-CGGGCGCU-UGCccAGGUCCUGC- -5' |
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| 16352 | 3' | -62.4 | NC_004084.1 | + | 9134 | 0.7 | 0.21645 |
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Target: 5'- uCGGCGUCCGuCGGGCGGaaGUCgAGGGg- -3' miRNA: 3'- -GCCGCGGGC-GCUUGCC--CAGgUCCUgc -5' |
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| 16352 | 3' | -62.4 | NC_004084.1 | + | 8197 | 0.66 | 0.432253 |
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Target: 5'- uGGgGUUCGCGGAUGGcGUCgAGGuuGa -3' miRNA: 3'- gCCgCGGGCGCUUGCC-CAGgUCCugC- -5' |
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| 16352 | 3' | -62.4 | NC_004084.1 | + | 7604 | 1.08 | 0.000307 |
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Target: 5'- cCGGCGCCCGCGAACGGGUCCAGGACGa -3' miRNA: 3'- -GCCGCGGGCGCUUGCCCAGGUCCUGC- -5' |
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| 16352 | 3' | -62.4 | NC_004084.1 | + | 7522 | 0.66 | 0.405701 |
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Target: 5'- -uGCGCCCGag--UGGGugcagccgaUCCAGGACGg -3' miRNA: 3'- gcCGCGGGCgcuuGCCC---------AGGUCCUGC- -5' |
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| 16352 | 3' | -62.4 | NC_004084.1 | + | 7239 | 0.68 | 0.324999 |
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Target: 5'- aGGauuUCCuCGAcUGGGUCCAGGACGa -3' miRNA: 3'- gCCgc-GGGcGCUuGCCCAGGUCCUGC- -5' |
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| 16352 | 3' | -62.4 | NC_004084.1 | + | 6754 | 0.66 | 0.38858 |
|
Target: 5'- aCGGCGuaaguCCCGCGGAagggcagucCGaGGUCUcggcgGGGACGu -3' miRNA: 3'- -GCCGC-----GGGCGCUU---------GC-CCAGG-----UCCUGC- -5' |
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| 16352 | 3' | -62.4 | NC_004084.1 | + | 6154 | 0.69 | 0.282608 |
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Target: 5'- cCGGgGUUCcugGCGAuCGGGcUCCAGGugGa -3' miRNA: 3'- -GCCgCGGG---CGCUuGCCC-AGGUCCugC- -5' |
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| 16352 | 3' | -62.4 | NC_004084.1 | + | 2839 | 0.73 | 0.151829 |
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Target: 5'- uCGGCGUCCGCGGugGa-UCgCAGGACa -3' miRNA: 3'- -GCCGCGGGCGCUugCccAG-GUCCUGc -5' |
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| 16352 | 3' | -62.4 | NC_004084.1 | + | 1568 | 0.99 | 0.001745 |
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Target: 5'- cCGGaGCCCGCGAACGGGUCCAGGACGa -3' miRNA: 3'- -GCCgCGGGCGCUUGCCCAGGUCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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