Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16355 | 3' | -55.4 | NC_004084.1 | + | 30133 | 0.71 | 0.455381 |
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Target: 5'- cGGGGAACUgCUCGAUC-UCcaUUCCg -3' miRNA: 3'- uCCCCUUGAgGAGCUAGuAGcgGAGG- -5' |
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| 16355 | 3' | -55.4 | NC_004084.1 | + | 53440 | 0.72 | 0.406077 |
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Target: 5'- gAGuGGGuccccgccgauCUCCUCGuggGUCGUCGUCUCCc -3' miRNA: 3'- -UC-CCCuu---------GAGGAGC---UAGUAGCGGAGG- -5' |
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| 16355 | 3' | -55.4 | NC_004084.1 | + | 35291 | 0.72 | 0.398794 |
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Target: 5'- uGGGGAGgUCCUCGAUCGuuUCGacggucaccUUUCCg -3' miRNA: 3'- uCCCCUUgAGGAGCUAGU--AGC---------GGAGG- -5' |
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| 16355 | 3' | -55.4 | NC_004084.1 | + | 46434 | 0.72 | 0.389808 |
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Target: 5'- uGGGGAACUCgUCGA--GUUGaCUCCa -3' miRNA: 3'- uCCCCUUGAGgAGCUagUAGCgGAGG- -5' |
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| 16355 | 3' | -55.4 | NC_004084.1 | + | 53521 | 0.72 | 0.380956 |
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Target: 5'- cGaGGAACUCCUCGua---CGCCUCCu -3' miRNA: 3'- uCcCCUUGAGGAGCuaguaGCGGAGG- -5' |
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| 16355 | 3' | -55.4 | NC_004084.1 | + | 22494 | 0.72 | 0.37571 |
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Target: 5'- cGGGGAcauGCucgugaaccgcgucaUCCUCGAcgacgAUCGCCUCCg -3' miRNA: 3'- uCCCCU---UG---------------AGGAGCUag---UAGCGGAGG- -5' |
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| 16355 | 3' | -55.4 | NC_004084.1 | + | 34862 | 0.75 | 0.27851 |
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Target: 5'- cGGGGAGCgcgauaucgUCCgaaUCGAUCAcgGCCUCCa -3' miRNA: 3'- uCCCCUUG---------AGG---AGCUAGUagCGGAGG- -5' |
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| 16355 | 3' | -55.4 | NC_004084.1 | + | 5727 | 0.75 | 0.258223 |
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Target: 5'- cGGGccGAGgUCCUCGAUCGggaugaUCGCCUCg -3' miRNA: 3'- uCCC--CUUgAGGAGCUAGU------AGCGGAGg -5' |
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| 16355 | 3' | -55.4 | NC_004084.1 | + | 15414 | 0.84 | 0.063913 |
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Target: 5'- uGGGGAACUCCUCGAUCG-CGUCgaguUCCu -3' miRNA: 3'- uCCCCUUGAGGAGCUAGUaGCGG----AGG- -5' |
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| 16355 | 3' | -55.4 | NC_004084.1 | + | 6752 | 1.11 | 0.00078 |
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Target: 5'- uAGGGGAACUCCUCGAUCAUCGCCUCCu -3' miRNA: 3'- -UCCCCUUGAGGAGCUAGUAGCGGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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