Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16356 | 3' | -52.9 | NC_004084.1 | + | 47947 | 0.66 | 0.833567 |
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Target: 5'- ----aGGCCGuccGUCAGGCCGUccuGGAu -3' miRNA: 3'- cauaaCUGGCca-CAGUCCGGCA---UCUu -5' |
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| 16356 | 3' | -52.9 | NC_004084.1 | + | 23096 | 0.66 | 0.824289 |
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Target: 5'- ----gGGCCGGUcGUCaAGGCCGgaaAGGu -3' miRNA: 3'- cauaaCUGGCCA-CAG-UCCGGCa--UCUu -5' |
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| 16356 | 3' | -52.9 | NC_004084.1 | + | 45795 | 0.66 | 0.814798 |
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Target: 5'- -gGUUGACCGaccgauccgGUCAGGCCGg---- -3' miRNA: 3'- caUAACUGGCca-------CAGUCCGGCaucuu -5' |
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| 16356 | 3' | -52.9 | NC_004084.1 | + | 13617 | 0.66 | 0.814798 |
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Target: 5'- ----cGACCGGgugGUCGGGaCCGUcguGAc -3' miRNA: 3'- cauaaCUGGCCa--CAGUCC-GGCAu--CUu -5' |
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| 16356 | 3' | -52.9 | NC_004084.1 | + | 19868 | 0.73 | 0.40831 |
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Target: 5'- ---cUGACCGGUGUU-GGuuGUAGAGu -3' miRNA: 3'- cauaACUGGCCACAGuCCggCAUCUU- -5' |
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| 16356 | 3' | -52.9 | NC_004084.1 | + | 6646 | 1.06 | 0.002501 |
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Target: 5'- gGUAUUGACCGGUGUCAGGCCGUAGAAg -3' miRNA: 3'- -CAUAACUGGCCACAGUCCGGCAUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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