Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16361 | 5' | -50.7 | NC_004084.1 | + | 35350 | 0.67 | 0.864774 |
Target: 5'- -aCCUCGucgcgucGCCGGCgacgaucgcGAAGUGCGc -3' miRNA: 3'- caGGAGCu------UGGCCGaaaa-----CUUCACGU- -5' |
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16361 | 5' | -50.7 | NC_004084.1 | + | 26831 | 0.68 | 0.849244 |
Target: 5'- --gCUUGAACCGGUg---GAAGUGUu -3' miRNA: 3'- cagGAGCUUGGCCGaaaaCUUCACGu -5' |
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16361 | 5' | -50.7 | NC_004084.1 | + | 18265 | 0.69 | 0.811975 |
Target: 5'- cGUCUUCGAACCGGUcgacgUUGAGGc--- -3' miRNA: 3'- -CAGGAGCUUGGCCGaa---AACUUCacgu -5' |
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16361 | 5' | -50.7 | NC_004084.1 | + | 53485 | 0.7 | 0.760919 |
Target: 5'- cGUCCUCGAG-CGGCUg--GAgccccucgacguAGUGCu -3' miRNA: 3'- -CAGGAGCUUgGCCGAaaaCU------------UCACGu -5' |
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16361 | 5' | -50.7 | NC_004084.1 | + | 1627 | 0.79 | 0.261503 |
Target: 5'- cUCCUCGAggaggccgACCGGCUUcugcgUGggGUGCu -3' miRNA: 3'- cAGGAGCU--------UGGCCGAAa----ACuuCACGu -5' |
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16361 | 5' | -50.7 | NC_004084.1 | + | 3932 | 1.1 | 0.002542 |
Target: 5'- cGUCCUCGAACCGGCUUUUGAAGUGCAg -3' miRNA: 3'- -CAGGAGCUUGGCCGAAAACUUCACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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