miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
16363 3' -61.2 NC_004084.1 + 12608 0.66 0.427667
Target:  5'- aGGGAacGAugUCCGGCCUgacCGGAUCg -3'
miRNA:   3'- gCCCUacCU--AGGCCGGGaccGCCUAG- -5'
16363 3' -61.2 NC_004084.1 + 9210 0.67 0.374989
Target:  5'- -uGGAUGGAUCCcGUCCUGGauGAc- -3'
miRNA:   3'- gcCCUACCUAGGcCGGGACCgcCUag -5'
16363 3' -61.2 NC_004084.1 + 31309 0.68 0.342393
Target:  5'- uGGGGuagcccuucUGGAUCCGGCUCgGGaucCGGAg- -3'
miRNA:   3'- gCCCU---------ACCUAGGCCGGGaCC---GCCUag -5'
16363 3' -61.2 NC_004084.1 + 10729 0.68 0.324598
Target:  5'- uCGGGGUcgguuuccgguuggGGAUCCGGCUCgccagUGGgGGcGUCg -3'
miRNA:   3'- -GCCCUA--------------CCUAGGCCGGG-----ACCgCC-UAG- -5'
16363 3' -61.2 NC_004084.1 + 55425 0.72 0.167045
Target:  5'- cCGGGG-GGGUCUGGCUCagUGGUGcGAUCu -3'
miRNA:   3'- -GCCCUaCCUAGGCCGGG--ACCGC-CUAG- -5'
16363 3' -61.2 NC_004084.1 + 2951 1.09 0.000276
Target:  5'- cCGGGAUGGAUCCGGCCCUGGCGGAUCa -3'
miRNA:   3'- -GCCCUACCUAGGCCGGGACCGCCUAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.