Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 16364 | 3' | -57.3 | NC_004084.1 | + | 36241 | 0.71 | 0.346624 |
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Target: 5'- cUCGccuACCGGGUCGacgUCCUCGAGuGCGCc- -3' miRNA: 3'- -AGU---UGGCUCAGU---GGGAGCUC-CGCGac -5' |
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| 16364 | 3' | -57.3 | NC_004084.1 | + | 37586 | 0.72 | 0.315049 |
|
Target: 5'- -gAGCCGAGgCACUCUCGGGgaGCGCUc -3' miRNA: 3'- agUUGGCUCaGUGGGAGCUC--CGCGAc -5' |
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| 16364 | 3' | -57.3 | NC_004084.1 | + | 21466 | 0.73 | 0.258544 |
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Target: 5'- -gAACCGGcUCGCCCUCGAGG-GCg- -3' miRNA: 3'- agUUGGCUcAGUGGGAGCUCCgCGac -5' |
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| 16364 | 3' | -57.3 | NC_004084.1 | + | 13749 | 0.74 | 0.210596 |
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Target: 5'- cCggUCGGcUCGCCCUCGAGgucGCGCUGg -3' miRNA: 3'- aGuuGGCUcAGUGGGAGCUC---CGCGAC- -5' |
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| 16364 | 3' | -57.3 | NC_004084.1 | + | 13850 | 0.74 | 0.233537 |
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Target: 5'- gCAGCCaGGcccGUCAUCgUUGAGGCGCUGg -3' miRNA: 3'- aGUUGG-CU---CAGUGGgAGCUCCGCGAC- -5' |
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| 16364 | 3' | -57.3 | NC_004084.1 | + | 36936 | 0.75 | 0.189622 |
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Target: 5'- gCAACCGAGgcgaucgcCGCCCUCGAGG-GCg- -3' miRNA: 3'- aGUUGGCUCa-------GUGGGAGCUCCgCGac -5' |
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| 16364 | 3' | -57.3 | NC_004084.1 | + | 2085 | 1.09 | 0.000663 |
|
Target: 5'- gUCAACCGAGUCACCCUCGAGGCGCUGg -3' miRNA: 3'- -AGUUGGCUCAGUGGGAGCUCCGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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