miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
16364 3' -57.3 NC_004084.1 + 21006 0.67 0.566804
Target:  5'- --uAUCGcgcaGGcCAUCCUCGAGGCGCg- -3'
miRNA:   3'- aguUGGC----UCaGUGGGAGCUCCGCGac -5'
16364 3' -57.3 NC_004084.1 + 17257 0.66 0.620521
Target:  5'- gCGGCCGgugcAGUCggcgauGCCCUCggugGAGGCGgUGg -3'
miRNA:   3'- aGUUGGC----UCAG------UGGGAG----CUCCGCgAC- -5'
16364 3' -57.3 NC_004084.1 + 48481 0.66 0.630247
Target:  5'- -uGACCGAGcaacUCGCugaggcuCgUCGAGGCGCUc -3'
miRNA:   3'- agUUGGCUC----AGUG-------GgAGCUCCGCGAc -5'
16364 3' -57.3 NC_004084.1 + 2610 0.66 0.631328
Target:  5'- gUCGACCGAuUC-CUCUCGuuccGGUGUUGg -3'
miRNA:   3'- -AGUUGGCUcAGuGGGAGCu---CCGCGAC- -5'
16364 3' -57.3 NC_004084.1 + 47899 0.66 0.631328
Target:  5'- gCAACgCGAcgGUCGCUCcgCGAGGUGCc- -3'
miRNA:   3'- aGUUG-GCU--CAGUGGGa-GCUCCGCGac -5'
16364 3' -57.3 NC_004084.1 + 48374 0.66 0.631328
Target:  5'- aUCGACCGugauaGGUCACCgUCGAcGCGg-- -3'
miRNA:   3'- -AGUUGGC-----UCAGUGGgAGCUcCGCgac -5'
16364 3' -57.3 NC_004084.1 + 28942 0.66 0.642136
Target:  5'- --cACCGccgCACCCUCGAGcGCGUc- -3'
miRNA:   3'- aguUGGCucaGUGGGAGCUC-CGCGac -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.