Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16364 | 3' | -57.3 | NC_004084.1 | + | 5148 | 0.68 | 0.483832 |
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Target: 5'- gCGACCGAcGUCGuCCCU-GAGGCaGCa- -3' miRNA: 3'- aGUUGGCU-CAGU-GGGAgCUCCG-CGac -5' |
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| 16364 | 3' | -57.3 | NC_004084.1 | + | 725 | 0.67 | 0.544567 |
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Target: 5'- uUCGuccCCGAGgagcaACUCUCGAcgucggaGGCGCUGg -3' miRNA: 3'- -AGUu--GGCUCag---UGGGAGCU-------CCGCGAC- -5' |
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| 16364 | 3' | -57.3 | NC_004084.1 | + | 17030 | 0.67 | 0.556183 |
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Target: 5'- gCGACCGAGcgucugCGCCCgUCGAugcGGUGaCUGa -3' miRNA: 3'- aGUUGGCUCa-----GUGGG-AGCU---CCGC-GAC- -5' |
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| 16364 | 3' | -57.3 | NC_004084.1 | + | 21006 | 0.67 | 0.566804 |
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Target: 5'- --uAUCGcgcaGGcCAUCCUCGAGGCGCg- -3' miRNA: 3'- aguUGGC----UCaGUGGGAGCUCCGCGac -5' |
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| 16364 | 3' | -57.3 | NC_004084.1 | + | 17257 | 0.66 | 0.620521 |
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Target: 5'- gCGGCCGgugcAGUCggcgauGCCCUCggugGAGGCGgUGg -3' miRNA: 3'- aGUUGGC----UCAG------UGGGAG----CUCCGCgAC- -5' |
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| 16364 | 3' | -57.3 | NC_004084.1 | + | 47899 | 0.66 | 0.631328 |
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Target: 5'- gCAACgCGAcgGUCGCUCcgCGAGGUGCc- -3' miRNA: 3'- aGUUG-GCU--CAGUGGGa-GCUCCGCGac -5' |
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| 16364 | 3' | -57.3 | NC_004084.1 | + | 36936 | 0.75 | 0.189622 |
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Target: 5'- gCAACCGAGgcgaucgcCGCCCUCGAGG-GCg- -3' miRNA: 3'- aGUUGGCUCa-------GUGGGAGCUCCgCGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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