Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16365 | 3' | -54.9 | NC_004084.1 | + | 42073 | 0.7 | 0.50921 |
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Target: 5'- -aGGCUgUGCGAaGCGCCGCgGUCUUCc -3' miRNA: 3'- gaCCGAaGUGCUgUGCGGCG-UAGAGG- -5' |
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| 16365 | 3' | -54.9 | NC_004084.1 | + | 8764 | 0.71 | 0.488591 |
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Target: 5'- -cGGCUUCAgcCGGUACGCCGa--CUCCa -3' miRNA: 3'- gaCCGAAGU--GCUGUGCGGCguaGAGG- -5' |
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| 16365 | 3' | -54.9 | NC_004084.1 | + | 47424 | 0.71 | 0.438876 |
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Target: 5'- -cGGCUcCuCGuCA-GCCGCAUCUCCu -3' miRNA: 3'- gaCCGAaGuGCuGUgCGGCGUAGAGG- -5' |
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| 16365 | 3' | -54.9 | NC_004084.1 | + | 1220 | 0.72 | 0.410471 |
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Target: 5'- gCUGGCUcggcggcgggaUCgaggGCGAuCGCGCC-CAUCUCCu -3' miRNA: 3'- -GACCGA-----------AG----UGCU-GUGCGGcGUAGAGG- -5' |
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| 16365 | 3' | -54.9 | NC_004084.1 | + | 40509 | 0.78 | 0.184345 |
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Target: 5'- -aGGCUguucgUACGACACGUCGgCGUCUUCa -3' miRNA: 3'- gaCCGAa----GUGCUGUGCGGC-GUAGAGG- -5' |
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| 16365 | 3' | -54.9 | NC_004084.1 | + | 1922 | 1.12 | 0.000777 |
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Target: 5'- cCUGGCUUCACGACACGCCGCAUCUCCu -3' miRNA: 3'- -GACCGAAGUGCUGUGCGGCGUAGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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