Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16365 | 5' | -58.5 | NC_004084.1 | + | 32097 | 0.67 | 0.534362 |
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Target: 5'- aCGGAuGGACgagcuCGAGcAGCuCGUCGUCGAGc -3' miRNA: 3'- -GCCU-UCUG-----GCUC-UCGcGCGGCAGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 51315 | 0.67 | 0.55398 |
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Target: 5'- aCGGc-GACCGAggcgaucGAGUGCGCcCG-CGAGa -3' miRNA: 3'- -GCCuuCUGGCU-------CUCGCGCG-GCaGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 31037 | 0.66 | 0.573807 |
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Target: 5'- gCGGAAG-CUGcGAGCGaaucccgcgccaGCCGUCGGu -3' miRNA: 3'- -GCCUUCuGGCuCUCGCg-----------CGGCAGCUc -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 7051 | 0.66 | 0.575904 |
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Target: 5'- aGGGAGGCCagcaGAGgaacAGCGUGuacCCGUCGAc -3' miRNA: 3'- gCCUUCUGG----CUC----UCGCGC---GGCAGCUc -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 3345 | 0.66 | 0.575904 |
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Target: 5'- aCGGggGA---GGAGCGCGCCGg---- -3' miRNA: 3'- -GCCuuCUggcUCUCGCGCGGCagcuc -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 52660 | 0.66 | 0.596961 |
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Target: 5'- gCGGcGAGACgGAGAu--CGUCGUCGAGg -3' miRNA: 3'- -GCC-UUCUGgCUCUcgcGCGGCAGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 29540 | 0.66 | 0.596961 |
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Target: 5'- cCGGu-GACgCGccGGAGCGUaagcagGCCGUUGAGg -3' miRNA: 3'- -GCCuuCUG-GC--UCUCGCG------CGGCAGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 29399 | 0.66 | 0.606476 |
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Target: 5'- uCGGgcGGCCacuccuGGGGCGUcgcgguuGCCGUCGAc -3' miRNA: 3'- -GCCuuCUGGc-----UCUCGCG-------CGGCAGCUc -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 18832 | 0.66 | 0.607534 |
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Target: 5'- aGGAGGACgGuG-GCGCGgcuuUCGUCGAc -3' miRNA: 3'- gCCUUCUGgCuCuCGCGC----GGCAGCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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