Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16365 | 5' | -58.5 | NC_004084.1 | + | 28912 | 0.7 | 0.382363 |
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Target: 5'- gCGGGuguGcCCGAGAGCGaCGUCGgccUCGGGa -3' miRNA: 3'- -GCCUu--CuGGCUCUCGC-GCGGC---AGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 17023 | 0.7 | 0.382363 |
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Target: 5'- gGGAuccgcGACCGAGcgucuGCGC-CCGUCGAu -3' miRNA: 3'- gCCUu----CUGGCUCu----CGCGcGGCAGCUc -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 19505 | 0.7 | 0.373845 |
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Target: 5'- aGG-AGcCCGGGAGCGaaCGCCGguUCGAGc -3' miRNA: 3'- gCCuUCuGGCUCUCGC--GCGGC--AGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 46313 | 0.7 | 0.349074 |
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Target: 5'- aGGAuGAUCGAGAGCGUGUcaccuuCGcCGAGu -3' miRNA: 3'- gCCUuCUGGCUCUCGCGCG------GCaGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 17973 | 0.73 | 0.237135 |
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Target: 5'- aCGGcGGaAUCGucGGCGCGCUGUCGAGc -3' miRNA: 3'- -GCCuUC-UGGCucUCGCGCGGCAGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 50594 | 0.73 | 0.231243 |
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Target: 5'- gCGGAu--CUGGGAGCGUGCCuccagcGUCGAGg -3' miRNA: 3'- -GCCUucuGGCUCUCGCGCGG------CAGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 24801 | 0.74 | 0.214305 |
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Target: 5'- -aGAGGACUGAucGUGCGCCGaUCGAGa -3' miRNA: 3'- gcCUUCUGGCUcuCGCGCGGC-AGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 1538 | 0.78 | 0.101988 |
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Target: 5'- uCGGucuGGACCGAG-GCGacgGCCGUCGAGc -3' miRNA: 3'- -GCCu--UCUGGCUCuCGCg--CGGCAGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 1960 | 1.09 | 0.000599 |
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Target: 5'- aCGGAAGACCGAGAGCGCGCCGUCGAGa -3' miRNA: 3'- -GCCUUCUGGCUCUCGCGCGGCAGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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