Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16365 | 5' | -58.5 | NC_004084.1 | + | 32097 | 0.67 | 0.534362 |
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Target: 5'- aCGGAuGGACgagcuCGAGcAGCuCGUCGUCGAGc -3' miRNA: 3'- -GCCU-UCUG-----GCUC-UCGcGCGGCAGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 33206 | 0.67 | 0.521081 |
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Target: 5'- uGGGAG-CCGAagacagaggcggccGAGCGgGCCGUCc-- -3' miRNA: 3'- gCCUUCuGGCU--------------CUCGCgCGGCAGcuc -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 46313 | 0.7 | 0.349074 |
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Target: 5'- aGGAuGAUCGAGAGCGUGUcaccuuCGcCGAGu -3' miRNA: 3'- gCCUuCUGGCUCUCGCGCG------GCaGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 49059 | 0.68 | 0.474239 |
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Target: 5'- uCGGAAGAUCGuGAccgucgacGUGCGaCgGUCGGGa -3' miRNA: 3'- -GCCUUCUGGCuCU--------CGCGC-GgCAGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 50594 | 0.73 | 0.231243 |
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Target: 5'- gCGGAu--CUGGGAGCGUGCCuccagcGUCGAGg -3' miRNA: 3'- -GCCUucuGGCUCUCGCGCGG------CAGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 51157 | 0.68 | 0.47132 |
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Target: 5'- aGGAGGcgaucgagacggucGCCGAGcaCGCGCCGaucgUCGAGg -3' miRNA: 3'- gCCUUC--------------UGGCUCucGCGCGGC----AGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 51315 | 0.67 | 0.55398 |
|
Target: 5'- aCGGc-GACCGAggcgaucGAGUGCGCcCG-CGAGa -3' miRNA: 3'- -GCCuuCUGGCU-------CUCGCGCG-GCaGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 52660 | 0.66 | 0.596961 |
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Target: 5'- gCGGcGAGACgGAGAu--CGUCGUCGAGg -3' miRNA: 3'- -GCC-UUCUGgCUCUcgcGCGGCAGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 56172 | 0.7 | 0.382363 |
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Target: 5'- aCGGcAAGACCGAGGagaucuccGCGCugGCCGaagCGAGc -3' miRNA: 3'- -GCC-UUCUGGCUCU--------CGCG--CGGCa--GCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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