Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16365 | 5' | -58.5 | NC_004084.1 | + | 27514 | 0.67 | 0.513983 |
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Target: 5'- -cGAGGACCuccuccgggGAGAuCGCGCCGuugUCGAGa -3' miRNA: 3'- gcCUUCUGG---------CUCUcGCGCGGC---AGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 33206 | 0.67 | 0.521081 |
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Target: 5'- uGGGAG-CCGAagacagaggcggccGAGCGgGCCGUCc-- -3' miRNA: 3'- gCCUUCuGGCU--------------CUCGCgCGGCAGcuc -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 6780 | 0.67 | 0.523116 |
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Target: 5'- gCGGAAcGCCGAagacgucGGGgaucagcuccCGCGCCGUCGAc -3' miRNA: 3'- -GCCUUcUGGCU-------CUC----------GCGCGGCAGCUc -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 16139 | 0.67 | 0.534362 |
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Target: 5'- aCGGguGACCGcGGuGGuCGCGCUGcUCGAGc -3' miRNA: 3'- -GCCuuCUGGC-UC-UC-GCGCGGC-AGCUC- -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 31037 | 0.66 | 0.573807 |
|
Target: 5'- gCGGAAG-CUGcGAGCGaaucccgcgccaGCCGUCGGu -3' miRNA: 3'- -GCCUUCuGGCuCUCGCg-----------CGGCAGCUc -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 7051 | 0.66 | 0.575904 |
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Target: 5'- aGGGAGGCCagcaGAGgaacAGCGUGuacCCGUCGAc -3' miRNA: 3'- gCCUUCUGG----CUC----UCGCGC---GGCAGCUc -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 29399 | 0.66 | 0.606476 |
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Target: 5'- uCGGgcGGCCacuccuGGGGCGUcgcgguuGCCGUCGAc -3' miRNA: 3'- -GCCuuCUGGc-----UCUCGCG-------CGGCAGCUc -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 18832 | 0.66 | 0.607534 |
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Target: 5'- aGGAGGACgGuG-GCGCGgcuuUCGUCGAc -3' miRNA: 3'- gCCUUCUGgCuCuCGCGC----GGCAGCUc -5' |
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| 16365 | 5' | -58.5 | NC_004084.1 | + | 1960 | 1.09 | 0.000599 |
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Target: 5'- aCGGAAGACCGAGAGCGCGCCGUCGAGa -3' miRNA: 3'- -GCCUUCUGGCUCUCGCGCGGCAGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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