Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16367 | 3' | -50.3 | NC_004084.1 | + | 50984 | 0.69 | 0.869831 |
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Target: 5'- cCGAGGUCuCG-CCACcGUcggcaucgcccUCGUCGUCGa -3' miRNA: 3'- -GCUUCAG-GCaGGUGcUA-----------AGUAGCAGC- -5' |
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| 16367 | 3' | -50.3 | NC_004084.1 | + | 36676 | 0.69 | 0.860841 |
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Target: 5'- gGAA--CCGUCCAgGGUUCGuggguccUCGUCGg -3' miRNA: 3'- gCUUcaGGCAGGUgCUAAGU-------AGCAGC- -5' |
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| 16367 | 3' | -50.3 | NC_004084.1 | + | 15087 | 0.72 | 0.726026 |
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Target: 5'- -cGAGaCCGUCCAC---UCGUCGUCGg -3' miRNA: 3'- gcUUCaGGCAGGUGcuaAGUAGCAGC- -5' |
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| 16367 | 3' | -50.3 | NC_004084.1 | + | 46541 | 0.72 | 0.715241 |
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Target: 5'- uCGAAGUCuucgaCGUCCG-GA-UCGUCGUCGg -3' miRNA: 3'- -GCUUCAG-----GCAGGUgCUaAGUAGCAGC- -5' |
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| 16367 | 3' | -50.3 | NC_004084.1 | + | 562 | 0.73 | 0.671378 |
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Target: 5'- uCGAGGUgaCGUUCGCGAU--GUCGUCGa -3' miRNA: 3'- -GCUUCAg-GCAGGUGCUAagUAGCAGC- -5' |
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| 16367 | 3' | -50.3 | NC_004084.1 | + | 36416 | 0.77 | 0.445273 |
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Target: 5'- uCGAuGUUCGUCCGCGcgUCGUCGaCGu -3' miRNA: 3'- -GCUuCAGGCAGGUGCuaAGUAGCaGC- -5' |
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| 16367 | 3' | -50.3 | NC_004084.1 | + | 768 | 1.1 | 0.004103 |
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Target: 5'- aCGAAGUCCGUCCACGAUUCAUCGUCGc -3' miRNA: 3'- -GCUUCAGGCAGGUGCUAAGUAGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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