Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16367 | 3' | -50.3 | NC_004084.1 | + | 41064 | 0.67 | 0.919558 |
Target: 5'- aGAAG-CgGUCCGCGAguaCGUCGaCGa -3' miRNA: 3'- gCUUCaGgCAGGUGCUaa-GUAGCaGC- -5' |
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16367 | 3' | -50.3 | NC_004084.1 | + | 46541 | 0.72 | 0.715241 |
Target: 5'- uCGAAGUCuucgaCGUCCG-GA-UCGUCGUCGg -3' miRNA: 3'- -GCUUCAG-----GCAGGUgCUaAGUAGCAGC- -5' |
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16367 | 3' | -50.3 | NC_004084.1 | + | 49736 | 0.66 | 0.959679 |
Target: 5'- -cGAGUCCucgGUCCACug--CGUCGUCa -3' miRNA: 3'- gcUUCAGG---CAGGUGcuaaGUAGCAGc -5' |
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16367 | 3' | -50.3 | NC_004084.1 | + | 50984 | 0.69 | 0.869831 |
Target: 5'- cCGAGGUCuCG-CCACcGUcggcaucgcccUCGUCGUCGa -3' miRNA: 3'- -GCUUCAG-GCaGGUGcUA-----------AGUAGCAGC- -5' |
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16367 | 3' | -50.3 | NC_004084.1 | + | 53867 | 0.66 | 0.967002 |
Target: 5'- cCGGAGUUCGUUUACGGUgagcaggccugCAUCGcCu -3' miRNA: 3'- -GCUUCAGGCAGGUGCUAa----------GUAGCaGc -5' |
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16367 | 3' | -50.3 | NC_004084.1 | + | 56583 | 0.68 | 0.89277 |
Target: 5'- aCGAAGaguucaCCGUCCGCaacugCAUCGUCu -3' miRNA: 3'- -GCUUCa-----GGCAGGUGcuaa-GUAGCAGc -5' |
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16367 | 3' | -50.3 | NC_004084.1 | + | 58119 | 0.69 | 0.877739 |
Target: 5'- uCGGGuUCuCGUCgGCGGU-CGUCGUCGa -3' miRNA: 3'- -GCUUcAG-GCAGgUGCUAaGUAGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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