Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16368 | 5' | -59.7 | NC_004084.1 | + | 9064 | 0.67 | 0.416308 |
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Target: 5'- uCGGGCGACgucGacgaUCACCUcgacacgaUCGCCGAu -3' miRNA: 3'- uGCCCGCUGac-Cg---AGUGGA--------AGCGGUU- -5' |
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| 16368 | 5' | -59.7 | NC_004084.1 | + | 7570 | 0.67 | 0.398094 |
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Target: 5'- uCGGcCGACUuGCUCgucucGCCUUCGCCGu -3' miRNA: 3'- uGCCcGCUGAcCGAG-----UGGAAGCGGUu -5' |
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| 16368 | 5' | -59.7 | NC_004084.1 | + | 51403 | 0.67 | 0.3804 |
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Target: 5'- cGCGGGCGcACUcGaUCGCCUcggUCGCCGu -3' miRNA: 3'- -UGCCCGC-UGAcCgAGUGGA---AGCGGUu -5' |
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| 16368 | 5' | -59.7 | NC_004084.1 | + | 51473 | 0.68 | 0.371753 |
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Target: 5'- uACGGGCGACgaccGGUUCcaucgacuCCUUCaCCAAg -3' miRNA: 3'- -UGCCCGCUGa---CCGAGu-------GGAAGcGGUU- -5' |
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| 16368 | 5' | -59.7 | NC_004084.1 | + | 49882 | 0.68 | 0.36324 |
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Target: 5'- uGCGGGaCGAUgggucuugUGGCUCAaccucggagauuCCUUCGCUGGa -3' miRNA: 3'- -UGCCC-GCUG--------ACCGAGU------------GGAAGCGGUU- -5' |
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| 16368 | 5' | -59.7 | NC_004084.1 | + | 2285 | 0.7 | 0.278704 |
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Target: 5'- uCGGGCGugUGGCcgugCAUCUU-GCCGu -3' miRNA: 3'- uGCCCGCugACCGa---GUGGAAgCGGUu -5' |
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| 16368 | 5' | -59.7 | NC_004084.1 | + | 19328 | 0.7 | 0.271849 |
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Target: 5'- gACGGGCGACUGuCUCACUcaagCGCg-- -3' miRNA: 3'- -UGCCCGCUGACcGAGUGGaa--GCGguu -5' |
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| 16368 | 5' | -59.7 | NC_004084.1 | + | 838 | 1.05 | 0.000644 |
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Target: 5'- gACGGGCGACUGGCUCACCUUCGCCAAc -3' miRNA: 3'- -UGCCCGCUGACCGAGUGGAAGCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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