miRNA: 3'- agaagGUGGCUcc-----AGUGGUCUGCUGG- -5'
miRNA: 3'- agaAGGUGGCUCCAGU--------GGU-CUGCUGG- -5'
miRNA: 3'- agaagGUGGCUCCAGuGGuCUGCUGG- -5'
miRNA: 3'- -AGAAGgUGG-C-UCc----AGUGGUCUGCUGG- -5'
miRNA: 3'- agaAGGUGG--CUCcAGUGGUCuGCUGg -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16371 | 5' | -54.6 | NC_004084.1 | + | 48173 | 0.69 | 0.590472 |
|
Target: 5'- ----aCGCCGAccgcgacUCACCAGGCGACCu -3' miRNA: 3'- agaagGUGGCUcc-----AGUGGUCUGCUGG- -5' |
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| 16371 | 5' | -54.6 | NC_004084.1 | + | 50988 | 0.73 | 0.417158 |
|
Target: 5'- --gUUCAUCGAGGUCGgugauaccCCGuGACGACCg -3' miRNA: 3'- agaAGGUGGCUCCAGU--------GGU-CUGCUGG- -5' |
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| 16371 | 5' | -54.6 | NC_004084.1 | + | 52348 | 0.66 | 0.800795 |
|
Target: 5'- ----gCGCCGGGGUU-CCuGGCGAUCg -3' miRNA: 3'- agaagGUGGCUCCAGuGGuCUGCUGG- -5' |
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| 16371 | 5' | -54.6 | NC_004084.1 | + | 54366 | 0.69 | 0.590472 |
|
Target: 5'- gUCUUCgGCCaGcAGaaugcUUACCAGACGGCCg -3' miRNA: 3'- -AGAAGgUGG-C-UCc----AGUGGUCUGCUGG- -5' |
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| 16371 | 5' | -54.6 | NC_004084.1 | + | 57497 | 0.66 | 0.7912 |
|
Target: 5'- --gUCCACCucGAGcUCGCCGG-CGACg -3' miRNA: 3'- agaAGGUGG--CUCcAGUGGUCuGCUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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