Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16378 | 5' | -55.3 | NC_004084.1 | + | 26964 | 0.66 | 0.747741 |
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Target: 5'- uGCCGcgaucgCCuCGGAGGAcauCGCG-CUACc -3' miRNA: 3'- uCGGCaa----GG-GCCUCCUu--GUGCaGAUG- -5' |
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| 16378 | 5' | -55.3 | NC_004084.1 | + | 2928 | 0.66 | 0.747741 |
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Target: 5'- aAGCUGggagaggagaUCCCGGAGGAcucACGCGaCUu- -3' miRNA: 3'- -UCGGCa---------AGGGCCUCCU---UGUGCaGAug -5' |
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| 16378 | 5' | -55.3 | NC_004084.1 | + | 5070 | 0.66 | 0.737346 |
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Target: 5'- cAGgUGUUCCgGGAGGGcggugaGCG-GUCUGCc -3' miRNA: 3'- -UCgGCAAGGgCCUCCU------UGUgCAGAUG- -5' |
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| 16378 | 5' | -55.3 | NC_004084.1 | + | 20670 | 0.66 | 0.705577 |
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Target: 5'- uGGCCc-UCCUGGAGGAugACcUCUuCa -3' miRNA: 3'- -UCGGcaAGGGCCUCCUugUGcAGAuG- -5' |
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| 16378 | 5' | -55.3 | NC_004084.1 | + | 29554 | 0.67 | 0.684023 |
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Target: 5'- cGCCGcaCCCuc--GAGCGCGUCUACg -3' miRNA: 3'- uCGGCaaGGGccucCUUGUGCAGAUG- -5' |
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| 16378 | 5' | -55.3 | NC_004084.1 | + | 44229 | 0.67 | 0.651351 |
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Target: 5'- uGGCCGa---CGGcuGGGAGCuCGUCUACg -3' miRNA: 3'- -UCGGCaaggGCC--UCCUUGuGCAGAUG- -5' |
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| 16378 | 5' | -55.3 | NC_004084.1 | + | 1704 | 0.68 | 0.629465 |
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Target: 5'- cGuCCGagUCCCagcGGAGGAugGCGgCUGCu -3' miRNA: 3'- uC-GGCa-AGGG---CCUCCUugUGCaGAUG- -5' |
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| 16378 | 5' | -55.3 | NC_004084.1 | + | 27714 | 0.7 | 0.480314 |
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Target: 5'- cGCUGUUCCCGGgugAGGAgccaggcgagacGCugGUCa-- -3' miRNA: 3'- uCGGCAAGGGCC---UCCU------------UGugCAGaug -5' |
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| 16378 | 5' | -55.3 | NC_004084.1 | + | 34823 | 0.7 | 0.470244 |
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Target: 5'- gAGCgcaGUUCCCGGcGGAGCACGa---- -3' miRNA: 3'- -UCGg--CAAGGGCCuCCUUGUGCagaug -5' |
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| 16378 | 5' | -55.3 | NC_004084.1 | + | 4591 | 1.1 | 0.000884 |
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Target: 5'- gAGCCGUUCCCGGAGGAACACGUCUACg -3' miRNA: 3'- -UCGGCAAGGGCCUCCUUGUGCAGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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