Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16379 | 5' | -60 | NC_004084.1 | + | 9147 | 0.66 | 0.489122 |
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Target: 5'- gGCggaaGUCGAGGGGUaaUCgCucauGCCGCUc -3' miRNA: 3'- gUGaug-CAGCUCCCCG--AG-Gu---CGGCGA- -5' |
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| 16379 | 5' | -60 | NC_004084.1 | + | 12060 | 0.66 | 0.459722 |
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Target: 5'- aACU-CGUCGAGuuGaCUCCAGuCCGCg -3' miRNA: 3'- gUGAuGCAGCUCccC-GAGGUC-GGCGa -5' |
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| 16379 | 5' | -60 | NC_004084.1 | + | 46266 | 0.66 | 0.459722 |
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Target: 5'- aGCU-CGUCGAGGaguuCUCCGGUCGUa -3' miRNA: 3'- gUGAuGCAGCUCCcc--GAGGUCGGCGa -5' |
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| 16379 | 5' | -60 | NC_004084.1 | + | 16177 | 0.67 | 0.440651 |
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Target: 5'- aGCUGagauUCaGuGGGGUacUCCAGCCGCUg -3' miRNA: 3'- gUGAUgc--AG-CuCCCCG--AGGUCGGCGA- -5' |
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| 16379 | 5' | -60 | NC_004084.1 | + | 29487 | 0.67 | 0.40123 |
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Target: 5'- gGgUGCGgCGGugguucuccagcuuGGGGCUCCAGCCaGCc -3' miRNA: 3'- gUgAUGCaGCU--------------CCCCGAGGUCGG-CGa -5' |
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| 16379 | 5' | -60 | NC_004084.1 | + | 36025 | 0.68 | 0.360799 |
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Target: 5'- gGCUGCGUgGuGGcGGCUCCAGuUCGg- -3' miRNA: 3'- gUGAUGCAgCuCC-CCGAGGUC-GGCga -5' |
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| 16379 | 5' | -60 | NC_004084.1 | + | 4923 | 1.06 | 0.000585 |
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Target: 5'- gCACUACGUCGAGGGGCUCCAGCCGCUc -3' miRNA: 3'- -GUGAUGCAGCUCCCCGAGGUCGGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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