Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16383 | 3' | -52.2 | NC_004084.1 | + | 7598 | 0.66 | 0.863338 |
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Target: 5'- uCGGGCcacaGCCAgCAGCUGGACUaCGa -3' miRNA: 3'- -GCUCGacc-UGGUaGUUGACCUGAaGC- -5' |
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| 16383 | 3' | -52.2 | NC_004084.1 | + | 6042 | 0.67 | 0.85495 |
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Target: 5'- cCGGcGCUGGGuuguaCAUCGcCUGGACgccgUCGg -3' miRNA: 3'- -GCU-CGACCUg----GUAGUuGACCUGa---AGC- -5' |
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| 16383 | 3' | -52.2 | NC_004084.1 | + | 53125 | 0.68 | 0.790025 |
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Target: 5'- cCGAGUggauggcGGACCAUCcGCUgcgGGACgucgUCGa -3' miRNA: 3'- -GCUCGa------CCUGGUAGuUGA---CCUGa---AGC- -5' |
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| 16383 | 3' | -52.2 | NC_004084.1 | + | 19007 | 0.68 | 0.779984 |
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Target: 5'- uCGAGUgaGGACgAcgacgccgagcUCgAACUGGACUUCGa -3' miRNA: 3'- -GCUCGa-CCUGgU-----------AG-UUGACCUGAAGC- -5' |
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| 16383 | 3' | -52.2 | NC_004084.1 | + | 36994 | 0.68 | 0.758398 |
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Target: 5'- aCGAGCUgaaguacGGucuCUcgCAGCUGGACgUCGa -3' miRNA: 3'- -GCUCGA-------CCu--GGuaGUUGACCUGaAGC- -5' |
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| 16383 | 3' | -52.2 | NC_004084.1 | + | 21603 | 0.7 | 0.650571 |
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Target: 5'- aGcAGCUGGACCGUCucg-GGACgaucUUCGa -3' miRNA: 3'- gC-UCGACCUGGUAGuugaCCUG----AAGC- -5' |
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| 16383 | 3' | -52.2 | NC_004084.1 | + | 2580 | 0.71 | 0.628223 |
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Target: 5'- gGAG-UGGGCCG---ACUGGACUUCa -3' miRNA: 3'- gCUCgACCUGGUaguUGACCUGAAGc -5' |
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| 16383 | 3' | -52.2 | NC_004084.1 | + | 5917 | 1.1 | 0.002071 |
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Target: 5'- aCGAGCUGGACCAUCAACUGGACUUCGu -3' miRNA: 3'- -GCUCGACCUGGUAGUUGACCUGAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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