Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16389 | 5' | -53.2 | NC_004084.1 | + | 11929 | 0.69 | 0.61635 |
Target: 5'- -cGGCGAcgucGCGaccagCGAGGACUCGAUCg -3' miRNA: 3'- caUCGCU----CGUcca--GUUCCUGAGCUAG- -5' |
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16389 | 5' | -53.2 | NC_004084.1 | + | 42736 | 0.69 | 0.638799 |
Target: 5'- --cGCGAGCAGGcgAAGGACaUCGGc- -3' miRNA: 3'- cauCGCUCGUCCagUUCCUG-AGCUag -5' |
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16389 | 5' | -53.2 | NC_004084.1 | + | 9109 | 1.11 | 0.001198 |
Target: 5'- aGUAGCGAGCAGGUCAAGGACUCGAUCg -3' miRNA: 3'- -CAUCGCUCGUCCAGUUCCUGAGCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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