Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16392 | 5' | -56.8 | NC_004084.1 | + | 22961 | 0.66 | 0.679628 |
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Target: 5'- -cUGGGuguUGGCCUCGAGUGUgaucUCACGg -3' miRNA: 3'- cuGCCU---GCCGGAGCUCGCAga--AGUGU- -5' |
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| 16392 | 5' | -56.8 | NC_004084.1 | + | 14450 | 0.66 | 0.658079 |
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Target: 5'- --gGGAUGGUCgCGAGUGUCagCACu -3' miRNA: 3'- cugCCUGCCGGaGCUCGCAGaaGUGu -5' |
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| 16392 | 5' | -56.8 | NC_004084.1 | + | 33404 | 0.66 | 0.655918 |
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Target: 5'- gGAuCGGGCGGCugCUCGAGauaggcuaucaaGUCUUgACAg -3' miRNA: 3'- -CU-GCCUGCCG--GAGCUCg-----------CAGAAgUGU- -5' |
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| 16392 | 5' | -56.8 | NC_004084.1 | + | 226 | 0.66 | 0.636437 |
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Target: 5'- -cUGGAUGGCCUCGgggugGGCcUCUUCGu- -3' miRNA: 3'- cuGCCUGCCGGAGC-----UCGcAGAAGUgu -5' |
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| 16392 | 5' | -56.8 | NC_004084.1 | + | 39929 | 0.67 | 0.603968 |
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Target: 5'- cGACGGcgccGCGGCCgccgauggacUCGAGCGcCaUCAUg -3' miRNA: 3'- -CUGCC----UGCCGG----------AGCUCGCaGaAGUGu -5' |
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| 16392 | 5' | -56.8 | NC_004084.1 | + | 50921 | 0.68 | 0.518989 |
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Target: 5'- aGCGGAuCGGCCUCGA-CGUCg--ACGa -3' miRNA: 3'- cUGCCU-GCCGGAGCUcGCAGaagUGU- -5' |
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| 16392 | 5' | -56.8 | NC_004084.1 | + | 31154 | 0.69 | 0.488286 |
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Target: 5'- cGCGGugGGCCggaCGA-CGUCUcagCGCAu -3' miRNA: 3'- cUGCCugCCGGa--GCUcGCAGAa--GUGU- -5' |
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| 16392 | 5' | -56.8 | NC_004084.1 | + | 20631 | 0.7 | 0.439132 |
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Target: 5'- --gGGAUGGCCgggCGGGUGUCggaUCGCc -3' miRNA: 3'- cugCCUGCCGGa--GCUCGCAGa--AGUGu -5' |
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| 16392 | 5' | -56.8 | NC_004084.1 | + | 24453 | 0.71 | 0.375349 |
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Target: 5'- gGACGGAguCGGCucgcaucucgaaCUCGAGCGUggUCGCGu -3' miRNA: 3'- -CUGCCU--GCCG------------GAGCUCGCAgaAGUGU- -5' |
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| 16392 | 5' | -56.8 | NC_004084.1 | + | 10482 | 1.08 | 0.000999 |
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Target: 5'- uGACGGACGGCCUCGAGCGUCUUCACAc -3' miRNA: 3'- -CUGCCUGCCGGAGCUCGCAGAAGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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