Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16395 | 3' | -58 | NC_004084.1 | + | 17553 | 0.66 | 0.560017 |
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Target: 5'- --aGC-GUCGAcgacAGCggAGCGUGGGACGc -3' miRNA: 3'- uagCGcCAGCU----UCG--UCGCACCCUGCu -5' |
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| 16395 | 3' | -58 | NC_004084.1 | + | 51333 | 0.67 | 0.507858 |
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Target: 5'- uUCGgGGUCGGucGCAGCGauccGGACGu -3' miRNA: 3'- uAGCgCCAGCUu-CGUCGCac--CCUGCu -5' |
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| 16395 | 3' | -58 | NC_004084.1 | + | 56778 | 0.67 | 0.506835 |
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Target: 5'- gGUCGCGG-CG-AGUcgAGCGUGGcguagauGACGAa -3' miRNA: 3'- -UAGCGCCaGCuUCG--UCGCACC-------CUGCU- -5' |
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| 16395 | 3' | -58 | NC_004084.1 | + | 40551 | 0.67 | 0.487565 |
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Target: 5'- cGUCGCGuUCGAGGCcGUGU-GGACGu -3' miRNA: 3'- -UAGCGCcAGCUUCGuCGCAcCCUGCu -5' |
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| 16395 | 3' | -58 | NC_004084.1 | + | 57656 | 0.68 | 0.447223 |
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Target: 5'- aAUCGUGGacggacuucgUCGAacgacuauacgaGGCGGCGUauaucgaGGGACGAg -3' miRNA: 3'- -UAGCGCC----------AGCU------------UCGUCGCA-------CCCUGCU- -5' |
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| 16395 | 3' | -58 | NC_004084.1 | + | 48563 | 0.69 | 0.419836 |
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Target: 5'- cGUCGCGGUCGAGGCGaaucaGGACGc -3' miRNA: 3'- -UAGCGCCAGCUUCGUcgcacCCUGCu -5' |
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| 16395 | 3' | -58 | NC_004084.1 | + | 29420 | 0.69 | 0.410631 |
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Target: 5'- -gCGCGaGuUCGAgAGC-GCGUGGGACGu -3' miRNA: 3'- uaGCGC-C-AGCU-UCGuCGCACCCUGCu -5' |
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| 16395 | 3' | -58 | NC_004084.1 | + | 29080 | 0.72 | 0.260567 |
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Target: 5'- cGUCGCGGUugccgucgacggCGAGcuucaccgcgaacGCGGCGUGGGcGCGAu -3' miRNA: 3'- -UAGCGCCA------------GCUU-------------CGUCGCACCC-UGCU- -5' |
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| 16395 | 3' | -58 | NC_004084.1 | + | 11185 | 1.06 | 0.000881 |
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Target: 5'- gAUCGCGGUCGAAGCAGCGUGGGACGAc -3' miRNA: 3'- -UAGCGCCAGCUUCGUCGCACCCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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