Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16399 | 5' | -57.9 | NC_004084.1 | + | 3024 | 0.66 | 0.610424 |
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Target: 5'- cGUCCCcGAcGUGGcGAauACGGCGAUGa -3' miRNA: 3'- -CAGGGuCU-CACC-CUgcUGUCGCUGCa -5' |
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| 16399 | 5' | -57.9 | NC_004084.1 | + | 7914 | 0.67 | 0.536109 |
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Target: 5'- cGUCCCAGAGcggaucUGGGAgCGugccucCAGCGuCGa -3' miRNA: 3'- -CAGGGUCUC------ACCCU-GCu-----GUCGCuGCa -5' |
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| 16399 | 5' | -57.9 | NC_004084.1 | + | 55949 | 0.67 | 0.505178 |
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Target: 5'- --aCCGGGGaccuucccGGGuACGACGGCGACGc -3' miRNA: 3'- cagGGUCUCa-------CCC-UGCUGUCGCUGCa -5' |
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| 16399 | 5' | -57.9 | NC_004084.1 | + | 7194 | 0.68 | 0.484989 |
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Target: 5'- -aCCgAGAGUGGGaucGCGAgCAGCG-CGa -3' miRNA: 3'- caGGgUCUCACCC---UGCU-GUCGCuGCa -5' |
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| 16399 | 5' | -57.9 | NC_004084.1 | + | 27456 | 0.68 | 0.47504 |
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Target: 5'- -aUCCGGAGcGGGA-GACGGuCGGCGUc -3' miRNA: 3'- caGGGUCUCaCCCUgCUGUC-GCUGCA- -5' |
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| 16399 | 5' | -57.9 | NC_004084.1 | + | 7344 | 0.68 | 0.449664 |
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Target: 5'- -cCCCAGAGUGGGgucaggccaucuACGAggaacagcagaagaaCAGCGACc- -3' miRNA: 3'- caGGGUCUCACCC------------UGCU---------------GUCGCUGca -5' |
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| 16399 | 5' | -57.9 | NC_004084.1 | + | 41734 | 0.75 | 0.167343 |
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Target: 5'- cGUCCCGGAugacgaucGGGACGACgccGGCGACGa -3' miRNA: 3'- -CAGGGUCUca------CCCUGCUG---UCGCUGCa -5' |
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| 16399 | 5' | -57.9 | NC_004084.1 | + | 12669 | 0.94 | 0.007739 |
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Target: 5'- cGUCCCAGAGUGGGACGACGaCGACGUu -3' miRNA: 3'- -CAGGGUCUCACCCUGCUGUcGCUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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