Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
164 | 3' | -55.6 | AC_000007.1 | + | 4214 | 0.66 | 0.585331 |
Target: 5'- gGAGGUAGcACCacUGCA-GAGCUUCaUGCu -3' miRNA: 3'- gCUCCAUU-UGG--ACGUcCUCGAGG-GCG- -5' |
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164 | 3' | -55.6 | AC_000007.1 | + | 22651 | 0.66 | 0.556254 |
Target: 5'- uGAuGGUGcgcAGCCUGUGGagugaaaacuucaccGAGCUgCCGCg -3' miRNA: 3'- gCU-CCAU---UUGGACGUC---------------CUCGAgGGCG- -5' |
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164 | 3' | -55.6 | AC_000007.1 | + | 8333 | 0.67 | 0.529794 |
Target: 5'- aGAuGG-GAGCUguccaugGUcuGGAGCUCCCGCg -3' miRNA: 3'- gCU-CCaUUUGGa------CGu-CCUCGAGGGCG- -5' |
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164 | 3' | -55.6 | AC_000007.1 | + | 26118 | 0.67 | 0.504881 |
Target: 5'- gGGGGUuuACCUggacccccaguccgGCgAGGAGCUcaacccaaucccCCCGCc -3' miRNA: 3'- gCUCCAuuUGGA--------------CG-UCCUCGA------------GGGCG- -5' |
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164 | 3' | -55.6 | AC_000007.1 | + | 13602 | 0.67 | 0.486796 |
Target: 5'- aGAGGc-GGCgCUGCgaaAGGAaaGCUUCCGCa -3' miRNA: 3'- gCUCCauUUG-GACG---UCCU--CGAGGGCG- -5' |
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164 | 3' | -55.6 | AC_000007.1 | + | 27499 | 0.67 | 0.486796 |
Target: 5'- aGGGGcgcAGCUUGCGGGcGGCUUUCGUc -3' miRNA: 3'- gCUCCau-UUGGACGUCC-UCGAGGGCG- -5' |
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164 | 3' | -55.6 | AC_000007.1 | + | 11333 | 0.67 | 0.476303 |
Target: 5'- gCGAGGgagagGAGCCcgaggagaUGCGGGAucgaaaGUUCCaCGCa -3' miRNA: 3'- -GCUCCa----UUUGG--------ACGUCCU------CGAGG-GCG- -5' |
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164 | 3' | -55.6 | AC_000007.1 | + | 23361 | 0.69 | 0.415885 |
Target: 5'- uGGGGcgAuuCCUGCAGGcGUaCCUGCa -3' miRNA: 3'- gCUCCa-UuuGGACGUCCuCGaGGGCG- -5' |
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164 | 3' | -55.6 | AC_000007.1 | + | 23301 | 0.7 | 0.34308 |
Target: 5'- -cAGGUAcGCCUGCAGGAaucGC-CCCa- -3' miRNA: 3'- gcUCCAUuUGGACGUCCU---CGaGGGcg -5' |
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164 | 3' | -55.6 | AC_000007.1 | + | 12007 | 0.71 | 0.310279 |
Target: 5'- uGAGcGUGAGCCgGCGGcgcGAGCUCagcgaCCGCg -3' miRNA: 3'- gCUC-CAUUUGGaCGUC---CUCGAG-----GGCG- -5' |
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164 | 3' | -55.6 | AC_000007.1 | + | 8371 | 0.71 | 0.310279 |
Target: 5'- -cAGGUcag---GCGGGAGCUCCUGCa -3' miRNA: 3'- gcUCCAuuuggaCGUCCUCGAGGGCG- -5' |
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164 | 3' | -55.6 | AC_000007.1 | + | 21430 | 0.72 | 0.266346 |
Target: 5'- gCGGGGUAAGCCUGUcccucgcgcaugguGGGAGCgagguagccuacgaaUCCUGa -3' miRNA: 3'- -GCUCCAUUUGGACG--------------UCCUCG---------------AGGGCg -5' |
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164 | 3' | -55.6 | AC_000007.1 | + | 8440 | 1.11 | 0.000385 |
Target: 5'- gCGAGGUAAACCUGCAGGAGCUCCCGCc -3' miRNA: 3'- -GCUCCAUUUGGACGUCCUCGAGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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