miRNA: 3'- cCUUGCUGC-AGCGGcCGCUCACCUGc -5'
miRNA: 3'- ccuUGCU-GCAGCGGCCGCU---------CACCUGC- -5'
miRNA: 3'- ccuuGCUGCAGCGGCCGCU-------CACCUGc -5'
miRNA: 3'- -CCUUGCUGCAGCGGCCGCUCACCUGC- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16401 | 3' | -57.1 | NC_004084.1 | + | 44957 | 0.78 | 0.153661 |
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Target: 5'- aGAACGGCGuUCGUC-GCGAGUGGACc -3' miRNA: 3'- cCUUGCUGC-AGCGGcCGCUCACCUGc -5' |
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| 16401 | 3' | -57.1 | NC_004084.1 | + | 23063 | 0.81 | 0.094544 |
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Target: 5'- --cGCGAuCGUCGCCGGCGAcgcgacgagGUGGACGu -3' miRNA: 3'- ccuUGCU-GCAGCGGCCGCU---------CACCUGC- -5' |
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| 16401 | 3' | -57.1 | NC_004084.1 | + | 57411 | 0.82 | 0.075629 |
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Target: 5'- ---uCGACGUCGCCGGCGAgcucgagGUGGACa -3' miRNA: 3'- ccuuGCUGCAGCGGCCGCU-------CACCUGc -5' |
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| 16401 | 3' | -57.1 | NC_004084.1 | + | 18143 | 1.11 | 0.000719 |
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Target: 5'- uGGAACGACGUCGCCGGCGAGUGGACGa -3' miRNA: 3'- -CCUUGCUGCAGCGGCCGCUCACCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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