Results 81 - 86 of 86 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16405 | 3' | -55.6 | NC_004084.1 | + | 55255 | 0.69 | 0.565761 |
Target: 5'- gUCGACGACGucuacgGUCGGCG-GCGugUagaucGUCa -3' miRNA: 3'- -AGCUGCUGC------UAGCUGCcCGCugA-----CAG- -5' |
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16405 | 3' | -55.6 | NC_004084.1 | + | 55900 | 0.71 | 0.483131 |
Target: 5'- gCGACGACGAUCcgccggagGAgUGGGCcGACUGg- -3' miRNA: 3'- aGCUGCUGCUAG--------CU-GCCCG-CUGACag -5' |
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16405 | 3' | -55.6 | NC_004084.1 | + | 55968 | 0.68 | 0.619274 |
Target: 5'- aCGACGGCGA-CG-CGGaGgGGCUGaUCg -3' miRNA: 3'- aGCUGCUGCUaGCuGCC-CgCUGAC-AG- -5' |
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16405 | 3' | -55.6 | NC_004084.1 | + | 56444 | 0.68 | 0.640813 |
Target: 5'- gCGaACGACGAucUCGACGGcGCG-CUcUCg -3' miRNA: 3'- aGC-UGCUGCU--AGCUGCC-CGCuGAcAG- -5' |
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16405 | 3' | -55.6 | NC_004084.1 | + | 56914 | 0.67 | 0.694358 |
Target: 5'- aUCGGCuuCGAgcUCGA-GGGCGAC-GUCu -3' miRNA: 3'- -AGCUGcuGCU--AGCUgCCCGCUGaCAG- -5' |
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16405 | 3' | -55.6 | NC_004084.1 | + | 58019 | 0.73 | 0.380125 |
Target: 5'- aUCGACGACGucugcgacAUCGGC-GGCGAC-GUCc -3' miRNA: 3'- -AGCUGCUGC--------UAGCUGcCCGCUGaCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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