Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16405 | 3' | -55.6 | NC_004084.1 | + | 16920 | 0.66 | 0.735186 |
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Target: 5'- aUCGugGcCGGgugcgguucggcaUCGGCGGGCGucGCgacGUCg -3' miRNA: 3'- -AGCugCuGCU-------------AGCUGCCCGC--UGa--CAG- -5' |
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| 16405 | 3' | -55.6 | NC_004084.1 | + | 41369 | 0.67 | 0.694358 |
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Target: 5'- gCGACGucagucacCGcAUCGACGGGCgcaGACgcucgGUCg -3' miRNA: 3'- aGCUGCu-------GC-UAGCUGCCCG---CUGa----CAG- -5' |
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| 16405 | 3' | -55.6 | NC_004084.1 | + | 37861 | 0.67 | 0.683723 |
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Target: 5'- uUCGaACGG-GAUgGcCGGGCGGgUGUCg -3' miRNA: 3'- -AGC-UGCUgCUAgCuGCCCGCUgACAG- -5' |
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| 16405 | 3' | -55.6 | NC_004084.1 | + | 7082 | 0.67 | 0.673041 |
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Target: 5'- gUCGACGAaGAUCucGCGGGCGcACUcgaucgccucgGUCg -3' miRNA: 3'- -AGCUGCUgCUAGc-UGCCCGC-UGA-----------CAG- -5' |
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| 16405 | 3' | -55.6 | NC_004084.1 | + | 653 | 0.67 | 0.673041 |
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Target: 5'- cUCGACGGCG-UCGugGuaGGUGuggaUGUCg -3' miRNA: 3'- -AGCUGCUGCuAGCugC--CCGCug--ACAG- -5' |
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| 16405 | 3' | -55.6 | NC_004084.1 | + | 50265 | 0.66 | 0.77647 |
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Target: 5'- gUUGGCGGCGAgguccUCGACGucugccgaccaGGCGuCgGUCa -3' miRNA: 3'- -AGCUGCUGCU-----AGCUGC-----------CCGCuGaCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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