Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 16408 | 3' | -57.2 | NC_004084.1 | + | 29136 | 0.67 | 0.573407 |
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Target: 5'- cCUCGgcgguGUCGGCGguGCUGGUGuGGUCGc -3' miRNA: 3'- aGAGU-----CAGCCGC--UGGCCGCuUCAGCa -5' |
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| 16408 | 3' | -57.2 | NC_004084.1 | + | 39841 | 0.67 | 0.573407 |
|
Target: 5'- gCUCgAGuccaUCGGCGGCCgcGGCGccGUCGa -3' miRNA: 3'- aGAG-UC----AGCCGCUGG--CCGCuuCAGCa -5' |
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| 16408 | 3' | -57.2 | NC_004084.1 | + | 54233 | 0.66 | 0.64835 |
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Target: 5'- aUCUCGacGUCGgaGCGAucagcgccgUCGGCGAGGUUGa -3' miRNA: 3'- -AGAGU--CAGC--CGCU---------GGCCGCUUCAGCa -5' |
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| 16408 | 3' | -57.2 | NC_004084.1 | + | 13252 | 0.66 | 0.64835 |
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Target: 5'- aCUCAccgucGUCGG-GAUCgauGGUGAAGUCGUu -3' miRNA: 3'- aGAGU-----CAGCCgCUGG---CCGCUUCAGCA- -5' |
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| 16408 | 3' | -57.2 | NC_004084.1 | + | 51240 | 0.66 | 0.626872 |
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Target: 5'- cUCUCGGUacugauaGGCGGCuucgucagCGGCGAAcgcuguGUCGUc -3' miRNA: 3'- -AGAGUCAg------CCGCUG--------GCCGCUU------CAGCA- -5' |
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| 16408 | 3' | -57.2 | NC_004084.1 | + | 43951 | 0.66 | 0.659072 |
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Target: 5'- gCUCAGgaGGCGGCCugggagGGCGAGGgaaCGa -3' miRNA: 3'- aGAGUCagCCGCUGG------CCGCUUCa--GCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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