Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16408 | 3' | -57.2 | NC_004084.1 | + | 49655 | 0.71 | 0.344746 |
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Target: 5'- ---gAGUCGGCGuACCGGCuGAAGcCGg -3' miRNA: 3'- agagUCAGCCGC-UGGCCG-CUUCaGCa -5' |
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| 16408 | 3' | -57.2 | NC_004084.1 | + | 46387 | 0.72 | 0.297959 |
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Target: 5'- cUUCAGgucgaaucgacgaUCGGCGA-CGGCGAAGUUGUu -3' miRNA: 3'- aGAGUC-------------AGCCGCUgGCCGCUUCAGCA- -5' |
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| 16408 | 3' | -57.2 | NC_004084.1 | + | 18976 | 0.73 | 0.257536 |
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Target: 5'- --cCAGUCGGCcGgCGGgGAAGUCGUa -3' miRNA: 3'- agaGUCAGCCGcUgGCCgCUUCAGCA- -5' |
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| 16408 | 3' | -57.2 | NC_004084.1 | + | 11662 | 0.74 | 0.238761 |
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Target: 5'- -gUCGGUCGGCGACgacuaCGGCGAGGacagCGa -3' miRNA: 3'- agAGUCAGCCGCUG-----GCCGCUUCa---GCa -5' |
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| 16408 | 3' | -57.2 | NC_004084.1 | + | 11915 | 0.77 | 0.148954 |
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Target: 5'- -aUCAacUCGGCGACCGGCGAcGUCGc -3' miRNA: 3'- agAGUc-AGCCGCUGGCCGCUuCAGCa -5' |
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| 16408 | 3' | -57.2 | NC_004084.1 | + | 20455 | 1.07 | 0.001094 |
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Target: 5'- gUCUCAGUCGGCGACCGGCGAAGUCGUc -3' miRNA: 3'- -AGAGUCAGCCGCUGGCCGCUUCAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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