Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16411 | 5' | -55 | NC_004084.1 | + | 16691 | 0.71 | 0.477057 |
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Target: 5'- -cGAUCGUCaucCGGGACGAUCUgaaggUCGAa -3' miRNA: 3'- acCUGGCAGa--GCCCUGCUAGA-----AGCUg -5' |
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| 16411 | 5' | -55 | NC_004084.1 | + | 1363 | 0.73 | 0.373921 |
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Target: 5'- cGGACCGUCgugaUCGGGACGcgaaagCGGCc -3' miRNA: 3'- aCCUGGCAG----AGCCCUGCuagaa-GCUG- -5' |
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| 16411 | 5' | -55 | NC_004084.1 | + | 7117 | 0.73 | 0.356857 |
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Target: 5'- cGGucGCCGUCUUGGuGAUGAUCUccguguagguguUCGGCc -3' miRNA: 3'- aCC--UGGCAGAGCC-CUGCUAGA------------AGCUG- -5' |
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| 16411 | 5' | -55 | NC_004084.1 | + | 5727 | 0.76 | 0.253057 |
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Target: 5'- cGGGCCGagguccucgaUCGGGAUGAUCgccUCGACg -3' miRNA: 3'- aCCUGGCag--------AGCCCUGCUAGa--AGCUG- -5' |
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| 16411 | 5' | -55 | NC_004084.1 | + | 21608 | 1.09 | 0.001264 |
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Target: 5'- cUGGACCGUCUCGGGACGAUCUUCGACu -3' miRNA: 3'- -ACCUGGCAGAGCCCUGCUAGAAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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