Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16414 | 5' | -53.6 | NC_004084.1 | + | 17250 | 0.66 | 0.844055 |
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Target: 5'- aGGAUCUGcGGcCGGUgcaGUCGGCgaUGCCc -3' miRNA: 3'- gCCUAGGC-CCaGCUA---UAGUCG--AUGGu -5' |
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| 16414 | 5' | -53.6 | NC_004084.1 | + | 45723 | 0.66 | 0.835247 |
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Target: 5'- gGGAUCCGacgCGGUgcugGUCGGCUACgAa -3' miRNA: 3'- gCCUAGGCccaGCUA----UAGUCGAUGgU- -5' |
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| 16414 | 5' | -53.6 | NC_004084.1 | + | 19715 | 0.67 | 0.788178 |
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Target: 5'- uCGGGcacUCCGGGUUGGUG-CAGCggUAgCAc -3' miRNA: 3'- -GCCU---AGGCCCAGCUAUaGUCG--AUgGU- -5' |
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| 16414 | 5' | -53.6 | NC_004084.1 | + | 49927 | 0.67 | 0.757891 |
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Target: 5'- uGGAUCCGGcGgcgcaGGUgGUCAGUgggGCCAg -3' miRNA: 3'- gCCUAGGCC-Cag---CUA-UAGUCGa--UGGU- -5' |
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| 16414 | 5' | -53.6 | NC_004084.1 | + | 3125 | 0.68 | 0.698481 |
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Target: 5'- aCGGggCUGGGaUCGAUAcgCAGCUgacgaucuacacgccGCCGa -3' miRNA: 3'- -GCCuaGGCCC-AGCUAUa-GUCGA---------------UGGU- -5' |
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| 16414 | 5' | -53.6 | NC_004084.1 | + | 18532 | 0.7 | 0.595131 |
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Target: 5'- gGGGUCCuGGUCGAUAccgcacugcagcUCAGCgUGCUc -3' miRNA: 3'- gCCUAGGcCCAGCUAU------------AGUCG-AUGGu -5' |
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| 16414 | 5' | -53.6 | NC_004084.1 | + | 18145 | 0.71 | 0.551524 |
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Target: 5'- uGGAgUCCGGGUCGA--UCAGCgcgAgCGu -3' miRNA: 3'- gCCU-AGGCCCAGCUauAGUCGa--UgGU- -5' |
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| 16414 | 5' | -53.6 | NC_004084.1 | + | 53384 | 0.71 | 0.540763 |
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Target: 5'- -aGAUCCGGcccucGUCGGg--CGGCUACCAa -3' miRNA: 3'- gcCUAGGCC-----CAGCUauaGUCGAUGGU- -5' |
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| 16414 | 5' | -53.6 | NC_004084.1 | + | 7163 | 0.71 | 0.530073 |
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Target: 5'- uGGuUCgGGGUCGGUcgCAGCgauCCGg -3' miRNA: 3'- gCCuAGgCCCAGCUAuaGUCGau-GGU- -5' |
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| 16414 | 5' | -53.6 | NC_004084.1 | + | 22566 | 1.09 | 0.001756 |
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Target: 5'- cCGGAUCCGGGUCGAUAUCAGCUACCAc -3' miRNA: 3'- -GCCUAGGCCCAGCUAUAGUCGAUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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