Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16418 | 3' | -57.2 | NC_004084.1 | + | 25469 | 0.7 | 0.417643 |
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Target: 5'- cGAGGUUCGCgGGCgGCgUCagaaggUGCGCGCCg -3' miRNA: 3'- -CUCCAAGUGgCUG-CG-AGg-----GCGUGUGG- -5' |
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| 16418 | 3' | -57.2 | NC_004084.1 | + | 27960 | 0.7 | 0.390573 |
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Target: 5'- cGGGGUUCACCcaccaGACGCgUgCGUucGCACCg -3' miRNA: 3'- -CUCCAAGUGG-----CUGCGaGgGCG--UGUGG- -5' |
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| 16418 | 3' | -57.2 | NC_004084.1 | + | 35333 | 0.71 | 0.348081 |
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Target: 5'- cGAGG-UCGCCGACGUccaccUCgUCGCGuCGCCg -3' miRNA: 3'- -CUCCaAGUGGCUGCG-----AG-GGCGU-GUGG- -5' |
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| 16418 | 3' | -57.2 | NC_004084.1 | + | 48545 | 0.72 | 0.316536 |
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Target: 5'- -cGG-UCGCCGACucuuccgccggGCUCCCGCGugaGCCg -3' miRNA: 3'- cuCCaAGUGGCUG-----------CGAGGGCGUg--UGG- -5' |
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| 16418 | 3' | -57.2 | NC_004084.1 | + | 50620 | 0.76 | 0.176482 |
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Target: 5'- cGGGUUCGCCGACGCUgCgGCGgggaagaACCa -3' miRNA: 3'- cUCCAAGUGGCUGCGAgGgCGUg------UGG- -5' |
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| 16418 | 3' | -57.2 | NC_004084.1 | + | 24167 | 1.11 | 0.000558 |
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Target: 5'- gGAGGUUCACCGACGCUCCCGCACACCu -3' miRNA: 3'- -CUCCAAGUGGCUGCGAGGGCGUGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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