Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16418 | 3' | -57.2 | NC_004084.1 | + | 16665 | 0.69 | 0.436313 |
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Target: 5'- gGAGGUccucgUCGCCGGCGUcgUCCCGaucguCAUCc -3' miRNA: 3'- -CUCCA-----AGUGGCUGCG--AGGGCgu---GUGG- -5' |
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| 16418 | 3' | -57.2 | NC_004084.1 | + | 14617 | 0.69 | 0.455454 |
|
Target: 5'- ----cUC-CCGGCGCUCCuCGC-CGCCg -3' miRNA: 3'- cuccaAGuGGCUGCGAGG-GCGuGUGG- -5' |
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| 16418 | 3' | -57.2 | NC_004084.1 | + | 14348 | 0.66 | 0.653455 |
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Target: 5'- cGAGGUaCugCGAggUGCUacgcggcaucUUCGCGCACCu -3' miRNA: 3'- -CUCCAaGugGCU--GCGA----------GGGCGUGUGG- -5' |
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| 16418 | 3' | -57.2 | NC_004084.1 | + | 7368 | 0.69 | 0.484989 |
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Target: 5'- -cGGUUCGcuCCGACGgugaUCCCGC-CugCg -3' miRNA: 3'- cuCCAAGU--GGCUGCg---AGGGCGuGugG- -5' |
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| 16418 | 3' | -57.2 | NC_004084.1 | + | 2302 | 0.67 | 0.554983 |
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Target: 5'- cGGGUcuggucgugcUCGCCGGagaucuuguugaGCUCCUGC-CGCCa -3' miRNA: 3'- cUCCA----------AGUGGCUg-----------CGAGGGCGuGUGG- -5' |
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| 16418 | 3' | -57.2 | NC_004084.1 | + | 1838 | 0.67 | 0.578306 |
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Target: 5'- --cGUUCACggUGugGCUCCUGCAgCGCg -3' miRNA: 3'- cucCAAGUG--GCugCGAGGGCGU-GUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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