Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16429 | 5' | -54.7 | NC_004084.1 | + | 41393 | 0.66 | 0.734913 |
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Target: 5'- gGCGCAgacGCUcgGUCGCGGAUC-CCa-- -3' miRNA: 3'- -UGCGU---UGAugCGGCGCCUAGaGGaaa -5' |
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| 16429 | 5' | -54.7 | NC_004084.1 | + | 31664 | 0.66 | 0.724199 |
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Target: 5'- gGCGuCAAUcccagggguagUACGCCGagauCGGGUCUCCa-- -3' miRNA: 3'- -UGC-GUUG-----------AUGCGGC----GCCUAGAGGaaa -5' |
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| 16429 | 5' | -54.7 | NC_004084.1 | + | 22468 | 0.66 | 0.702501 |
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Target: 5'- cGCGaCGGCUGCGUgGUGGcggCUCCa-- -3' miRNA: 3'- -UGC-GUUGAUGCGgCGCCua-GAGGaaa -5' |
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| 16429 | 5' | -54.7 | NC_004084.1 | + | 13904 | 0.66 | 0.702501 |
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Target: 5'- cUGCAGCUGgGCgaccgcuuCGCGGAaCUCCUc- -3' miRNA: 3'- uGCGUUGAUgCG--------GCGCCUaGAGGAaa -5' |
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| 16429 | 5' | -54.7 | NC_004084.1 | + | 15767 | 0.67 | 0.669455 |
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Target: 5'- gACGUcgucgGACUGCGCCGCcgacuGGAaCUCCc-- -3' miRNA: 3'- -UGCG-----UUGAUGCGGCG-----CCUaGAGGaaa -5' |
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| 16429 | 5' | -54.7 | NC_004084.1 | + | 14622 | 0.68 | 0.602678 |
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Target: 5'- gGCGCucCU-CGCCGcCGGAcUCUCCg-- -3' miRNA: 3'- -UGCGuuGAuGCGGC-GCCU-AGAGGaaa -5' |
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| 16429 | 5' | -54.7 | NC_004084.1 | + | 51811 | 0.72 | 0.368595 |
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Target: 5'- gUGguACUgcGCGCCGCaGAUCUCCUc- -3' miRNA: 3'- uGCguUGA--UGCGGCGcCUAGAGGAaa -5' |
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| 16429 | 5' | -54.7 | NC_004084.1 | + | 54455 | 0.72 | 0.368595 |
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Target: 5'- uGCGCcgcACUGuCGCCGCGGAUCgUCUUc -3' miRNA: 3'- -UGCGu--UGAU-GCGGCGCCUAGaGGAAa -5' |
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| 16429 | 5' | -54.7 | NC_004084.1 | + | 36696 | 0.73 | 0.34286 |
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Target: 5'- cUGCGAUgACGUCGCGGAUCUCg--- -3' miRNA: 3'- uGCGUUGaUGCGGCGCCUAGAGgaaa -5' |
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| 16429 | 5' | -54.7 | NC_004084.1 | + | 34404 | 1.04 | 0.00222 |
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Target: 5'- cACGCAACUACGCCGCGGAUCUCCUUUc -3' miRNA: 3'- -UGCGUUGAUGCGGCGCCUAGAGGAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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