Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
1644 | 3' | -56.3 | NC_001347.2 | + | 185023 | 0.71 | 0.779394 |
Target: 5'- gAUCUCGUGGggGuccaguucgGCCGgCGcGCGCUu -3' miRNA: 3'- -UAGAGCGCCuuCua-------UGGCaGC-CGCGG- -5' |
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1644 | 3' | -56.3 | NC_001347.2 | + | 134339 | 0.71 | 0.773988 |
Target: 5'- ---cCGCGGAcucgccucggccaggGGGUACCGaggCGGUGCCc -3' miRNA: 3'- uagaGCGCCU---------------UCUAUGGCa--GCCGCGG- -5' |
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1644 | 3' | -56.3 | NC_001347.2 | + | 161646 | 0.71 | 0.751953 |
Target: 5'- uGUCgaccCGCGGcguGGAcgcGCCGUUGGCGUCg -3' miRNA: 3'- -UAGa---GCGCCu--UCUa--UGGCAGCCGCGG- -5' |
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1644 | 3' | -56.3 | NC_001347.2 | + | 192179 | 0.71 | 0.733151 |
Target: 5'- cGUCUC-CGGAuGAgcgGCCG-CGGCGCg -3' miRNA: 3'- -UAGAGcGCCUuCUa--UGGCaGCCGCGg -5' |
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1644 | 3' | -56.3 | NC_001347.2 | + | 79051 | 0.72 | 0.723623 |
Target: 5'- ---cCGCGaGAAG--GCgCGUCGGCGCCa -3' miRNA: 3'- uagaGCGC-CUUCuaUG-GCAGCCGCGG- -5' |
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1644 | 3' | -56.3 | NC_001347.2 | + | 159550 | 0.72 | 0.692679 |
Target: 5'- ---aCGCGGuugaAAGGUACCGgcgguguuggagCGGCGCCa -3' miRNA: 3'- uagaGCGCC----UUCUAUGGCa-----------GCCGCGG- -5' |
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1644 | 3' | -56.3 | NC_001347.2 | + | 128978 | 1.1 | 0.003632 |
Target: 5'- cAUCUCGCGGAAGAUACCGUCGGCGCCg -3' miRNA: 3'- -UAGAGCGCCUUCUAUGGCAGCCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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