Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16444 | 3' | -53.7 | NC_004084.1 | + | 7604 | 0.7 | 0.58166 |
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Target: 5'- cACAGCCAGCAgCUGGacuacgaCGGCgaAGCUGGc -3' miRNA: 3'- aUGUUGGUUGU-GGCCa------GUCG--UCGACC- -5' |
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| 16444 | 3' | -53.7 | NC_004084.1 | + | 21586 | 0.7 | 0.570598 |
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Target: 5'- ---cACCGAUgcaACCGGcgAGCAGCUGGa -3' miRNA: 3'- auguUGGUUG---UGGCCagUCGUCGACC- -5' |
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| 16444 | 3' | -53.7 | NC_004084.1 | + | 51772 | 0.7 | 0.53667 |
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Target: 5'- cUACGGCCuGACACCGGUCaauaccgAGUGGC-GGu -3' miRNA: 3'- -AUGUUGG-UUGUGGCCAG-------UCGUCGaCC- -5' |
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| 16444 | 3' | -53.7 | NC_004084.1 | + | 52833 | 0.71 | 0.484606 |
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Target: 5'- gACAGCCAcCACCGuacagCGGCAGUUGa -3' miRNA: 3'- aUGUUGGUuGUGGCca---GUCGUCGACc -5' |
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| 16444 | 3' | -53.7 | NC_004084.1 | + | 6143 | 0.72 | 0.464051 |
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Target: 5'- cGCGACCAGCGCCGGgguuccuggcgaUCGGgcucCAGgUGGa -3' miRNA: 3'- aUGUUGGUUGUGGCC------------AGUC----GUCgACC- -5' |
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| 16444 | 3' | -53.7 | NC_004084.1 | + | 47101 | 0.73 | 0.405301 |
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Target: 5'- gACAACC--CGCCGGUCGGCAcCUGc -3' miRNA: 3'- aUGUUGGuuGUGGCCAGUCGUcGACc -5' |
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| 16444 | 3' | -53.7 | NC_004084.1 | + | 8131 | 0.75 | 0.295319 |
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Target: 5'- gAUGACCGACGCCuGGUCGGCAGaCgucgaGGa -3' miRNA: 3'- aUGUUGGUUGUGG-CCAGUCGUC-Ga----CC- -5' |
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| 16444 | 3' | -53.7 | NC_004084.1 | + | 38555 | 1.08 | 0.001513 |
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Target: 5'- cUACAACCAACACCGGUCAGCAGCUGGu -3' miRNA: 3'- -AUGUUGGUUGUGGCCAGUCGUCGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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