Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16445 | 3' | -54.3 | NC_004084.1 | + | 3323 | 0.66 | 0.7749 |
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Target: 5'- cCAGgAG-GAGCGCgCGUC--UCGGACg -3' miRNA: 3'- aGUCgUCaCUCGUG-GCAGacAGUCUG- -5' |
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| 16445 | 3' | -54.3 | NC_004084.1 | + | 38811 | 0.67 | 0.667528 |
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Target: 5'- aCGGguGUGAguGCAUUGUUUG-CGGGCg -3' miRNA: 3'- aGUCguCACU--CGUGGCAGACaGUCUG- -5' |
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| 16445 | 3' | -54.3 | NC_004084.1 | + | 58129 | 0.69 | 0.599588 |
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Target: 5'- gUCGGCGGUcgucgucGAGcCGCCGUgaGUguGGCa -3' miRNA: 3'- -AGUCGUCA-------CUC-GUGGCAgaCAguCUG- -5' |
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| 16445 | 3' | -54.3 | NC_004084.1 | + | 23609 | 0.69 | 0.5896 |
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Target: 5'- --cGCGGUGAGCA-CGUCg--CAGACc -3' miRNA: 3'- aguCGUCACUCGUgGCAGacaGUCUG- -5' |
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| 16445 | 3' | -54.3 | NC_004084.1 | + | 5084 | 0.69 | 0.556559 |
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Target: 5'- -gGGCGGUGAGCG--GUCUGccCAGGCg -3' miRNA: 3'- agUCGUCACUCGUggCAGACa-GUCUG- -5' |
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| 16445 | 3' | -54.3 | NC_004084.1 | + | 38648 | 1.09 | 0.001129 |
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Target: 5'- gUCAGCAGUGAGCACCGUCUGUCAGACc -3' miRNA: 3'- -AGUCGUCACUCGUGGCAGACAGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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