Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
16446 | 5' | -56.3 | NC_004084.1 | + | 43969 | 0.66 | 0.669322 |
Target: 5'- gAGGGCGAGGGa-ACGACGcucuacCGCGa-- -3' miRNA: 3'- -UCCCGCUCCUgaUGCUGUa-----GCGCagc -5' |
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16446 | 5' | -56.3 | NC_004084.1 | + | 34572 | 0.66 | 0.669322 |
Target: 5'- gAGGGCGcuggcGGcGACUGCcucCAUCGCGaCGg -3' miRNA: 3'- -UCCCGC-----UC-CUGAUGcu-GUAGCGCaGC- -5' |
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16446 | 5' | -56.3 | NC_004084.1 | + | 21868 | 0.66 | 0.680033 |
Target: 5'- ---uCGAGGACUGCGucauccGCAUCGCGagGa -3' miRNA: 3'- ucccGCUCCUGAUGC------UGUAGCGCagC- -5' |
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16446 | 5' | -56.3 | NC_004084.1 | + | 16138 | 0.66 | 0.6907 |
Target: 5'- -cGGUGAuGGACgaacugcGCGuCGUCGCGUCc -3' miRNA: 3'- ucCCGCU-CCUGa------UGCuGUAGCGCAGc -5' |
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16446 | 5' | -56.3 | NC_004084.1 | + | 10598 | 0.66 | 0.6907 |
Target: 5'- cGGGCGAaaGGuucggauacaccGCUGauCGACAacUCGCGUCu -3' miRNA: 3'- uCCCGCU--CC------------UGAU--GCUGU--AGCGCAGc -5' |
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16446 | 5' | -56.3 | NC_004084.1 | + | 36672 | 0.66 | 0.716061 |
Target: 5'- -cGGCagcaucgauguccagGAGGACUGCGAUgacGUCGCGgaucUCGu -3' miRNA: 3'- ucCCG---------------CUCCUGAUGCUG---UAGCGC----AGC- -5' |
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16446 | 5' | -56.3 | NC_004084.1 | + | 54567 | 0.66 | 0.722335 |
Target: 5'- gAGGGCGAgcugacucagcaGGACUACcACAccUCGCuuuUCGa -3' miRNA: 3'- -UCCCGCU------------CCUGAUGcUGU--AGCGc--AGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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