Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16446 | 5' | -56.3 | NC_004084.1 | + | 4023 | 0.7 | 0.45 |
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Target: 5'- -aGGCGucGACUggaACGGCgAUCGCGUCa -3' miRNA: 3'- ucCCGCucCUGA---UGCUG-UAGCGCAGc -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 43663 | 0.71 | 0.406907 |
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Target: 5'- cGGGCGGGccuaccgcgccgaguGcCUGCGACGgacgggCGCGUCGa -3' miRNA: 3'- uCCCGCUC---------------CuGAUGCUGUa-----GCGCAGC- -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 52641 | 0.71 | 0.385475 |
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Target: 5'- cAGGGUGAGugccucGGCUGCGGCGagacggagaUCGuCGUCGa -3' miRNA: 3'- -UCCCGCUC------CUGAUGCUGU---------AGC-GCAGC- -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 326 | 0.71 | 0.385475 |
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Target: 5'- aGGGGCGcggcgaccAGGACggcaACGACAaCGcCGUCGa -3' miRNA: 3'- -UCCCGC--------UCCUGa---UGCUGUaGC-GCAGC- -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 5353 | 0.8 | 0.112355 |
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Target: 5'- gGGcGGCGAGGACUGgGACGaucUCGaCGUCGa -3' miRNA: 3'- -UC-CCGCUCCUGAUgCUGU---AGC-GCAGC- -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 11680 | 0.81 | 0.095124 |
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Target: 5'- -cGGCGAGGACaGCGACGUCuacacgGCGUCGg -3' miRNA: 3'- ucCCGCUCCUGaUGCUGUAG------CGCAGC- -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 39315 | 1.08 | 0.001093 |
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Target: 5'- gAGGGCGAGGACUACGACAUCGCGUCGu -3' miRNA: 3'- -UCCCGCUCCUGAUGCUGUAGCGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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