Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16446 | 5' | -56.3 | NC_004084.1 | + | 43969 | 0.66 | 0.669322 |
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Target: 5'- gAGGGCGAGGGa-ACGACGcucuacCGCGa-- -3' miRNA: 3'- -UCCCGCUCCUgaUGCUGUa-----GCGCagc -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 49241 | 0.67 | 0.614383 |
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Target: 5'- cAGGGCGAuGACUGaagagauCGACGUCcaCGUCa -3' miRNA: 3'- -UCCCGCUcCUGAU-------GCUGUAGc-GCAGc -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 49776 | 0.68 | 0.603618 |
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Target: 5'- -cGGCGAGGACUACaGCcucgguguucucgAUCGCGaagUCGu -3' miRNA: 3'- ucCCGCUCCUGAUGcUG-------------UAGCGC---AGC- -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 50267 | 0.7 | 0.469504 |
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Target: 5'- uGGcGGCGAGGucCU-CGACGUCugccgaccagGCGUCGg -3' miRNA: 3'- -UC-CCGCUCCu-GAuGCUGUAG----------CGCAGC- -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 51209 | 0.69 | 0.540848 |
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Target: 5'- -uGGCGcGGGCUACugGGCGUggUGCGUCGa -3' miRNA: 3'- ucCCGCuCCUGAUG--CUGUA--GCGCAGC- -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 52641 | 0.71 | 0.385475 |
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Target: 5'- cAGGGUGAGugccucGGCUGCGGCGagacggagaUCGuCGUCGa -3' miRNA: 3'- -UCCCGCUC------CUGAUGCUGU---------AGC-GCAGC- -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 54567 | 0.66 | 0.722335 |
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Target: 5'- gAGGGCGAgcugacucagcaGGACUACcACAccUCGCuuuUCGa -3' miRNA: 3'- -UCCCGCU------------CCUGAUGcUGU--AGCGc--AGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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