Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16446 | 5' | -56.3 | NC_004084.1 | + | 16928 | 0.67 | 0.626242 |
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Target: 5'- cGGGuGCGGuucggcaucggcGGGCgucGCGACGUCGgGUCu -3' miRNA: 3'- -UCC-CGCU------------CCUGa--UGCUGUAGCgCAGc -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 856 | 0.67 | 0.62732 |
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Target: 5'- uGGGUGuGGAaagagucagucguCGACGUCGcCGUCGg -3' miRNA: 3'- uCCCGCuCCUgau----------GCUGUAGC-GCAGC- -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 42683 | 0.67 | 0.652119 |
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Target: 5'- cGGcGCGAGGcugccaGCgaucaccgaugcugACGACccuGUCGCGUCGg -3' miRNA: 3'- uCC-CGCUCC------UGa-------------UGCUG---UAGCGCAGC- -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 34572 | 0.66 | 0.669322 |
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Target: 5'- gAGGGCGcuggcGGcGACUGCcucCAUCGCGaCGg -3' miRNA: 3'- -UCCCGC-----UC-CUGAUGcu-GUAGCGCaGC- -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 21868 | 0.66 | 0.680033 |
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Target: 5'- ---uCGAGGACUGCGucauccGCAUCGCGagGa -3' miRNA: 3'- ucccGCUCCUGAUGC------UGUAGCGCagC- -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 36672 | 0.66 | 0.716061 |
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Target: 5'- -cGGCagcaucgauguccagGAGGACUGCGAUgacGUCGCGgaucUCGu -3' miRNA: 3'- ucCCG---------------CUCCUGAUGCUG---UAGCGC----AGC- -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 4023 | 0.7 | 0.45 |
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Target: 5'- -aGGCGucGACUggaACGGCgAUCGCGUCa -3' miRNA: 3'- ucCCGCucCUGA---UGCUG-UAGCGCAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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