Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16446 | 5' | -56.3 | NC_004084.1 | + | 51209 | 0.69 | 0.540848 |
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Target: 5'- -uGGCGcGGGCUACugGGCGUggUGCGUCGa -3' miRNA: 3'- ucCCGCuCCUGAUG--CUGUA--GCGCAGC- -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 36067 | 0.68 | 0.55136 |
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Target: 5'- uGGGGauacgaCGAGGACcGCGACGaggUCGcCGUCu -3' miRNA: 3'- -UCCC------GCUCCUGaUGCUGU---AGC-GCAGc -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 19935 | 0.68 | 0.602542 |
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Target: 5'- gAGGGCGAGGAUccguucaacccCGACAcggucUCGCGauccUCGa -3' miRNA: 3'- -UCCCGCUCCUGau---------GCUGU-----AGCGC----AGC- -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 20012 | 0.68 | 0.608998 |
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Target: 5'- cGGGGaauccgaucggcgcuCGAGGACgACGA--UCGCGUCu -3' miRNA: 3'- -UCCC---------------GCUCCUGaUGCUguAGCGCAGc -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 49241 | 0.67 | 0.614383 |
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Target: 5'- cAGGGCGAuGACUGaagagauCGACGUCcaCGUCa -3' miRNA: 3'- -UCCCGCUcCUGAU-------GCUGUAGc-GCAGc -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 26292 | 0.67 | 0.637028 |
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Target: 5'- --cGCGAGGuCUGuCGACcagAUCGUGUCGu -3' miRNA: 3'- uccCGCUCCuGAU-GCUG---UAGCGCAGC- -5' |
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| 16446 | 5' | -56.3 | NC_004084.1 | + | 39315 | 1.08 | 0.001093 |
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Target: 5'- gAGGGCGAGGACUACGACAUCGCGUCGu -3' miRNA: 3'- -UCCCGCUCCUGAUGCUGUAGCGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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