Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16453 | 5' | -51.1 | NC_004084.1 | + | 53572 | 0.66 | 0.93371 |
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Target: 5'- uGugGAGgugCCGGAugugcucGCGAcGAUCGGCg- -3' miRNA: 3'- -CugCUCaa-GGCCU-------UGCU-CUAGUUGgc -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 39773 | 0.66 | 0.934254 |
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Target: 5'- cGACGccgaagaCGGcGACGAGGUCGGCCa -3' miRNA: 3'- -CUGCucaag--GCC-UUGCUCUAGUUGGc -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 12384 | 0.66 | 0.939542 |
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Target: 5'- aGGCGAGggUCCGGuuAACGuccuGAUCGAUg- -3' miRNA: 3'- -CUGCUCa-AGGCC--UUGCu---CUAGUUGgc -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 43014 | 0.66 | 0.939542 |
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Target: 5'- cGAgGAGUUCCccAACGAGAcgCAAgCGg -3' miRNA: 3'- -CUgCUCAAGGccUUGCUCUa-GUUgGC- -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 46720 | 0.66 | 0.939542 |
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Target: 5'- cGACGAGUggUCGG-GCGAGcacAUCGAUCu -3' miRNA: 3'- -CUGCUCAa-GGCCuUGCUC---UAGUUGGc -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 22335 | 0.66 | 0.939542 |
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Target: 5'- cGACGAGgaacUCGaGGGCGAGAaCuACCGg -3' miRNA: 3'- -CUGCUCaa--GGC-CUUGCUCUaGuUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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