Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16453 | 5' | -51.1 | NC_004084.1 | + | 29785 | 0.74 | 0.508266 |
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Target: 5'- cGACGGGUcggcguacaccuacUCCGGccgcuACGAGAUCA-CCGa -3' miRNA: 3'- -CUGCUCA--------------AGGCCu----UGCUCUAGUuGGC- -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 2065 | 0.75 | 0.480032 |
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Target: 5'- gGugGA--UCCGugaGGACGAGGUCAACCGa -3' miRNA: 3'- -CugCUcaAGGC---CUUGCUCUAGUUGGC- -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 36075 | 0.75 | 0.45962 |
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Target: 5'- cGACGAGgaCCGcGACGAGGUC-GCCGu -3' miRNA: 3'- -CUGCUCaaGGCcUUGCUCUAGuUGGC- -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 55365 | 0.76 | 0.449588 |
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Target: 5'- cGACGAGUUCCucgaGGGugGcGAUCAucGCCGu -3' miRNA: 3'- -CUGCUCAAGG----CCUugCuCUAGU--UGGC- -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 42322 | 0.76 | 0.439679 |
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Target: 5'- cGACGAccgUCGGGACGAGAUCGACg- -3' miRNA: 3'- -CUGCUcaaGGCCUUGCUCUAGUUGgc -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 40992 | 1.11 | 0.002611 |
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Target: 5'- cGACGAGUUCCGGAACGAGAUCAACCGg -3' miRNA: 3'- -CUGCUCAAGGCCUUGCUCUAGUUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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