Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16453 | 5' | -51.1 | NC_004084.1 | + | 7853 | 0.68 | 0.85682 |
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Target: 5'- gGACGAGUUCUGG-ACGGacauGAUCGAggagacuCCGc -3' miRNA: 3'- -CUGCUCAAGGCCuUGCU----CUAGUU-------GGC- -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 5507 | 0.68 | 0.840351 |
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Target: 5'- cGACGAGUcguUCCuaguGGGugGCGAGGaacUCGACCa -3' miRNA: 3'- -CUGCUCA---AGG----CCU--UGCUCU---AGUUGGc -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 5356 | 0.69 | 0.822138 |
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Target: 5'- cGGCGAGgaCUGGGACGAucUCGACg- -3' miRNA: 3'- -CUGCUCaaGGCCUUGCUcuAGUUGgc -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 5041 | 0.67 | 0.903559 |
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Target: 5'- cGACGAGggCCGGAucuUGuagcGGUCGGCgGa -3' miRNA: 3'- -CUGCUCaaGGCCUu--GCu---CUAGUUGgC- -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 2065 | 0.75 | 0.480032 |
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Target: 5'- gGugGA--UCCGugaGGACGAGGUCAACCGa -3' miRNA: 3'- -CugCUcaAGGC---CUUGCUCUAGUUGGC- -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 1107 | 0.69 | 0.822138 |
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Target: 5'- --gGAGUUCUGGAA-GAuGGUCGAUCGg -3' miRNA: 3'- cugCUCAAGGCCUUgCU-CUAGUUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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