Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16453 | 5' | -51.1 | NC_004084.1 | + | 35412 | 0.67 | 0.903559 |
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Target: 5'- cGACGGGUcaucgucgucgaUCuCGcucGCGAGGUCGACCa -3' miRNA: 3'- -CUGCUCA------------AG-GCcu-UGCUCUAGUUGGc -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 9875 | 0.66 | 0.921014 |
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Target: 5'- cGACGAGguucuugaacccugcCCGGGAgaaccCGAGAUCGaacccGCCGa -3' miRNA: 3'- -CUGCUCaa-------------GGCCUU-----GCUCUAGU-----UGGC- -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 47909 | 0.66 | 0.916686 |
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Target: 5'- uGAUGAGcUCaCGGAGCGuuAUCGugUGa -3' miRNA: 3'- -CUGCUCaAG-GCCUUGCucUAGUugGC- -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 50133 | 0.66 | 0.922826 |
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Target: 5'- cGCGAucgUCCGGAugACGAGGUCGuugUCGa -3' miRNA: 3'- cUGCUca-AGGCCU--UGCUCUAGUu--GGC- -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 53572 | 0.66 | 0.93371 |
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Target: 5'- uGugGAGgugCCGGAugugcucGCGAcGAUCGGCg- -3' miRNA: 3'- -CugCUCaa-GGCCU-------UGCU-CUAGUUGgc -5' |
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| 16453 | 5' | -51.1 | NC_004084.1 | + | 55365 | 0.76 | 0.449588 |
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Target: 5'- cGACGAGUUCCucgaGGGugGcGAUCAucGCCGu -3' miRNA: 3'- -CUGCUCAAGG----CCUugCuCUAGU--UGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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