Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 16458 | 5' | -58.2 | NC_004084.1 | + | 396 | 0.74 | 0.211453 |
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Target: 5'- uCACC-UCACCGAgaGCGAGCAGGaGAu -3' miRNA: 3'- -GUGGcAGUGGCUagCGCUCGUCCgCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 19376 | 0.76 | 0.15847 |
|
Target: 5'- --aCGUC-CCGAUCGCaucgaccuGGGCAGGCGAu -3' miRNA: 3'- gugGCAGuGGCUAGCG--------CUCGUCCGCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 10667 | 0.77 | 0.134903 |
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Target: 5'- uGCCGUUugCGAUCGcCGGGCAG-CGAc -3' miRNA: 3'- gUGGCAGugGCUAGC-GCUCGUCcGCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 55986 | 0.79 | 0.097177 |
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Target: 5'- gGCUGaUCGCCGAUgGCGAGCAucgcuGGCGAg -3' miRNA: 3'- gUGGC-AGUGGCUAgCGCUCGU-----CCGCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 42004 | 0.79 | 0.091948 |
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Target: 5'- aCGCCGUCuuugcucgagugGCCG-UCGCGAGCgAGGCGGu -3' miRNA: 3'- -GUGGCAG------------UGGCuAGCGCUCG-UCCGCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 16121 | 0.81 | 0.062182 |
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Target: 5'- gAUCGUCGCCGAUCGUGAaCGGGUGAc -3' miRNA: 3'- gUGGCAGUGGCUAGCGCUcGUCCGCU- -5' |
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| 16458 | 5' | -58.2 | NC_004084.1 | + | 42722 | 1.08 | 0.000708 |
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Target: 5'- uCACCGUCACCGAUCGCGAGCAGGCGAa -3' miRNA: 3'- -GUGGCAGUGGCUAGCGCUCGUCCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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